Preliminary report on PCR diagnosis of Neosporosis in cattle in Hovd, Mongolia

WU Lijiang;DAO ErJiala;HAO Yunwei;GANG Temuer;MA Yu;BA Yinchahan;College of Veterinary Medicine, Xinjiang Agricultural University;University of Hovd;

Hovd;;cattle;;Neospora caninum;;PCR;;preliminary report

This study was to determine whether Neospora caninum had infected cattle in the province of Hovd, causing abortion risk. DNA extraction, amplification diagnosis and gene sequencing were performed on 120 cattle blood samples collected at three experimental sites in Kobuduo Province using PCR with Neospora NC-5 as the diagnostic target gene. The results showed that a 231 bp target fragment was successfully obtained. The sequencing results were compared by BLAST, and the homology of the present strain with the Neospora caninum New Zealand strain(GenBank accession number: AY459289.1) was 99%, and the constructed phylogenetic tree showed that the present strain and Neospora caninum were in one cluster, at a confidence level of 99%, indicating that the cattle in the province of Hovd had been infected with Neospora caninum. Among the three detection sites, the infection rate of Eldente Sumu Town was the highest, at 12.7%(7/55), with the total infection rate being 7.5%(9/120). It was estimated that Neosporosis had infected the cattle herds in the region at varying degrees, resulting in abortion of the cows. This was the first report on infection in cattle in Hovd, Mongolia with Neospora caninum; andmuch attention should be paid to more prevention and control of the disease.