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Position: Home > Articles > Tagging Blast Resistance Gene Pi 1 in Rice (Oryza sativa) Using Candidate Resistance Genes Rice Science 2004,11 (5/6) 251-254

Tagging Blast Resistance Gene Pi 1 in Rice (Oryza sativa) Using Candidate Resistance Genes

作者：

Wu Jian-li;Menchu BERNADO;ZHUANG Jie-yun;ZHENG Kang-le;Hei LEUNG

单位：

State Key Laboratory of Rice Biology;Chinese National Center for Rice Improvement; China National Rice Research Institute; China; Hangzhou 310006

关键词：

rice;candidate gene;marker-aided selection;blast resistance

摘要：

An F_3 population derived from C101LAC/CO39 containing 90 lines was analyzed for blast resistance with 48 candidate genes developed from resistance gene analogs (RGA) and suppression subtractive library. Genetic analysis confirmed that blast resistance of the population was controlled by a single gene Pi 1. One of the candidate'genes, R10 was identified as associated with the blast resistance gene on the long arm of chromosome 11 and mapped using a DH population derived from Azucena/IR64. A pair of PCR based primers was designed based on the sequence of R10 for marker-aided selection of the blast resistance gene. The recombination frequency between Pi 1 and the marker was estimated as 1.28%. It suggested that strategy of employing candidate genes is useful for gene identification and mapping: A new RFLP marker and the corresponding PCR marker for tagging of Pi 1 were provided.

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Tagging Blast Resistance Gene Pi 1 in Rice (Oryza sativa) Using Candidate Resistance Genes

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