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Position: Home > Articles > SRAP Analysis of Genetic Variation among Native Poplars in Southwest China Journal of Plant Genetic Resources 2015,16 (4) 836-841

西南地区乡土杨树遗传变异的SRAP分析

作  者:
员涛;颜璐茜;李佳蔓;周安佩;纵丹;李旦;于雷;何承忠
单  位:
云南省高校林木遗传改良与繁育重点实验室/西南地区生物多样性保育国家林业局重点实验室/西南山地森林资源保育与利用省部共建教育部重点实验室/西南林业大学;西南林业大学云南生物多样性研究院
关键词:
乡土杨树;遗传多样性;SRAP标记;西南地区
摘  要:
采用SRAP分子标记技术对西南地区11种乡土杨树共333份样本的遗传变异进行分析,7对引物组合共扩增出215条带,其中多态性条带158条,多态性条带百分率为73.49%,表明11种乡土杨树间存在广泛变异。AMOVA分析结果显示,种间遗传变异分量为10.84,占总变异的48.70%,遗传差异达极显著水平(P<0.001)。种间的遗传相似系数变幅在0.8199~0.9607之间,平均遗传相似系数为0.8983。聚类结果表明,昌都杨和藏川杨之间的遗传差异最小,大叶杨和三脉青杨之间的遗传差异最大。本研究结果为西南地区乡土杨树基因资源的保护、开发和利用提供了一定的科学理论依据。
译  名:
SRAP Analysis of Genetic Variation among Native Poplars in Southwest China
作  者:
YUN Tao;YAN Lu-xi;LI Jia-man;ZHOU An-pei;ZONG Dan;LI Dan;YU Lei;HE Cheng-zhong;Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province/Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration/Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education/Southwest Forestry University;Yunnan Academy of Biodiversity,Southwest Forestry University;
关键词:
native poplars;;genetic variation;;SRAP markers;;southwest China
摘  要:
333 samples of native poplars among 11 species collected from southwest China were examined by SRAP markers to determine the genetic variation. The results showed that 7 selected primer combinations of SRAP generated 215 bands,of which 158 bands were polymorphic. The percentage of polymorphic bands was 73. 49%. All these index indicated that there were considerable genetic variation existed among 11 native poplar species. The variance components among species was 10. 84 and it accounted for 48. 70% of the total genetic variation by molecular variance analysis( AMOVA) indicating the genetic difference among 11 native poplar species was significant( P <0. 001). The genetic similarity coefficients among the tested poplars ranged from 0. 8199 to 0. 9607,with an average of 0. 8983. The cluster analysis showed that there was the smallest genetic difference between Populus qamdoensis and P. szechuanica var. tibetica,whereas the largest genetic difference between P. lasiocarpa and P. trinervis. The results of this research would provide a scientific basis for conservation,development and utilization of the native poplar gene resources in southwest China.

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