当前位置: 首页 > 文章 > 几起兔腹泻疫情的诊断研究 山东农业大学学报(自然科学版) 2008,39 (3) 350-354
Position: Home > Articles > DIAGNOSTIC RESEARCH OF FIVE EPIDEMIC SITUATIONS OF RABBITS DIARRHEA Journal of Shandong Agricultural University(Natural Science Edition) 2008,39 (3) 350-354

几起兔腹泻疫情的诊断研究

作  者:
王海荣;LIU Si-dang;陈勇;柴同杰
单  位:
山东农业大学动物科技学院;平邑县畜牧局
关键词:
兔腹泻;A型魏氏梭菌;分离鉴定;多重聚合酶链反应(multi-PCR)。
摘  要:
对山东省泰安等地的5起兔的腹泻疫情进行临床诊断,根据初步诊断结果进行了病原的分离培养,可疑菌经多重PCR鉴定为A型魏氏梭菌。根据疫情报爆发时出现的临床特征,结合实验室诊断结果,可以确定上述5起兔的腹泻疫情与兔魏氏梭菌感染密切相关,分离的魏氏梭菌的的血清型为A型。
译  名:
DIAGNOSTIC RESEARCH OF FIVE EPIDEMIC SITUATIONS OF RABBITS DIARRHEA
作  者:
WANG Hai-rong1,LIUSi-dang1,CHENYong2,CHAI Tong-jie1*(1.Department of Animal Husbandry and Veterinary Medicine,Shandong Agricultural University,Taian 271018,China;2.Aanimal husbandary Department of Pingyi,Pingyi 250400,China)
关键词:
Diarrhea of rabbits;clostridium perfringens type A;isolation and genotyping;multiple PCR
摘  要:
Five epidemic situations of rabbit's diarrhea in Shangdong province were diagnosed by clinical signs and gross lesions.Fifteen grampositive rod bacteria were isolated from rabbits body with culture method.The isolateswere identified and genotypedby multiple PCR as to be clostridium welchii type A.combined with histro-pathological change and PCR amplification of rabbit's tissue DNA to αtoxin gene.,It showed that the five epidemic situations of rabbit,s diarrhea werecloselyrelated with infections of clostridium welchii type A.

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