Position: Home > Articles > Study on Immune Response of TGEV S Gene Expressed in Recombinant Lactobacillus with Mice
Chinese Journal of Veterinary Drug
2011,45
(4)
1-4
猪传染性胃肠炎病毒S基因重组乳酸菌免疫小鼠的研究
作 者：
刘义;陈中秋;董丽娜;胡桂学
单 位：
吉林农业大学动物科学技术学院
关键词：
猪传染性胃肠炎病毒S基因;重组乳酸菌;小鼠
摘 要：
将含有猪传染性胃肠炎病毒S基因的重组乳酸菌PNZ8149/NZ3900口服(灌胃)免疫BALB/C小鼠,第3次加强免疫后10 d捕杀小鼠,采集血液、脾脏、粪便,处理后采用T淋巴细胞增殖试验及间接ELISA方法,检查免疫小鼠对外源基因表达产物的应答情况。结果表明,含有S基因的重组乳酸菌经口服免疫小鼠后,血清中的IgG、粪便中的sIgA与对照组有明显的差异(P<0.05)。结果说明,含有猪传染性胃肠炎病毒S基因的重组乳酸菌,可以诱导小鼠产生良好的针对猪传染性胃肠炎病毒的全身体液和黏膜免疫应答。
译 名：
Study on Immune Response of TGEV S Gene Expressed in Recombinant Lactobacillus with Mice
作 者：
LIU Yi,CHEN Zhong-qiu,DONG Li-na,HU Gui-xue (College of Animal Science and Technology,Jilin Agriculture University,Changchun 130118;China)
关键词：
S gene of transmissible gastroenteritis virus;recombined lactobacillus;mice
摘 要：
TGEV S gene recombined lactobacillus,as oral vaccine,was lavaged to inoculate BALB/C mice.The mouse were killed after third time booster immunization,while blood,spleen,excrement were collected.The immune responses of expressing exogenous gene in immunization mice were checked up by thymus lymphopoiesis test and indirect ELISA.The result showed,differences were obvious on IgG in blood serum and sIgA in fecess between control group and test group,which took S gene recombined lactobacillus orally.Thus humor and mucous membrane response for TGEV were induced by the lactobacillus which was recombined S gene of TGEV.
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