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当前位置: 首页 > 文章 > 多壁碳纳米管分散固相萃取结合LC-MS-MS测定鸡肉中金刚烷胺 肉类研究 2014 (4) 14-18
Position: Home > Articles > Determination of Amantadine in Chicken Muscle by Liquid Chromatography with Tandem Mass Spectrometry Coupled with Dispersive Solid Phase Extraction Using Multiwalled Carbon Nanotubes as Adsorbent MEAT RESEARCH 2014 (4) 14-18

多壁碳纳米管分散固相萃取结合LC-MS-MS测定鸡肉中金刚烷胺

作  者：
孙亚米;刘勇军;赵健;叶宇飞;吕燕;吴银良
单  位：
宁波市农业科学研究院;中国动物疫病预防控制中心
关键词：
金刚烷胺;液相色谱串联质谱法;多壁碳纳米管;分散固相萃取
摘  要：
建立了采用多壁碳纳米管为吸附剂的分散固相萃取净化、液相色谱串联质谱测定鸡肉中金刚烷胺残留量的方法。鸡肉样品经乙酸乙腈提取后,调节pH值至11,加入75 mg多壁碳纳米管进行分散固相萃取,被吸附的药物经5.0%甲酸溶液:甲醇(5∶5,V/V)洗脱,洗脱液过滤膜后直接进样分析。采用Acquity UPLC BEH C18色谱柱分离,以0.1%甲酸水溶液和甲醇作为流动相作梯度洗脱,电喷雾正电子(ESI+)模式电离,多反应监测模式检测,内标法校准进行定量。金刚烷胺在0.05~5.0μg/L质量浓度范围内呈良好的线性,线性相关系数均大于0.99,鸡肉样品中最低定量限为0.50μg/kg。鸡肉样品中添加0.5~1.0μg/kg金刚烷胺的回收率在97.8%~103.6%之间,相对标准偏差均小于10%。
译  名：
Determination of Amantadine in Chicken Muscle by Liquid Chromatography with Tandem Mass Spectrometry Coupled with Dispersive Solid Phase Extraction Using Multiwalled Carbon Nanotubes as Adsorbent
作  者：
SUN Ya-mi;LIU Yong-jun;ZHAO Jian;YE Yu-fei;L Yan;WU Yin-liang;Ningbo Academy of Agricultural Sciences;The Center for Animal Disease Control and Prevention;
关键词：
amantadine;;LC-MS-MS;;MWCNTs;;dispersive solid phase extraction
摘  要：
A simple,sensitive and reliable analytical method was developed for amantadine in chicken muscle by ultra high performance liquid chromatography-positive electrospray ionisation tandem mass spectrometry(UHPLC-ESI-MS/MS) using dispersive solid phase extraction(dSPE) with multi-walled carbon nanotubes(MWCNTs).Chicken muscle was extracted using 1.0% acetic acid in acetonitrile.After adjusting pH value to 11,75 mg of MWCNTs were added to absorpt amantadine and 5.0% formic acid solution:methanol(5:5,V/V) was used to elute the compound from the MWCNTs.The eluate was directly analyzed by LC-MS-MS on an Acquity UPLC BEH C18 column with a mixture of methanol and 0.1% formic acid solution as the mobile phase.The samples were quantifi ed with the internal standard calibration curve method.Good linearities were obtained for amantadine in the concentration range of 0.05–5.0 μg/L with correlation coeffi cient more than 0.999.The recoveries for amantadine in chicken muscle were 97.8%–103.6% at three fortifi ed levels(0.5,0.75 and 1.0 μg/kg) with relative standard deviations less than 10.0%(n = 3).The limit of quantitation was 0.5 μg/kg.

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