单 位：
承德职业学院;中国农业科学院畜牧研究所;西北农林科技大学
关键词：
五指山小型猪近交系;白细胞抗原;经典SLAⅠ3基因;RT-PCR
摘 要：
【目的】为探讨五指山小型猪(WZSP)近交系群体中SLAⅠ3(SLA-3)位点等位基因分布结构及其特性。【方法】利用4对引物,采用RT-PCR扩增了32头WZSPSLA-3基因部分外显子1、外显子2和大部分外显子3序列。根据家系和扩增结果,选择8头个体的PCR产物克隆测序。【结果】获得9个不同的核苷酸序列。与GenBank中所用SLA-3基因的核苷酸序列及其编码的氨基酸序列进行比较分析,确定这9个序列均为SLA-3位点上的新等位基因,但这些等位基因间核苷酸变异很少;氨基酸序列比较发现猪和人之间有很高的保守性。同时,构建了23个SLA-3的等位基因和1个HLA-A等位基因核苷酸序列的系统树。【结论】WZSP在SLA-3位点和其它猪品种有明显的差异,拥有其独特的遗传资源。从分子水平为WZSPSLA-3基因的分子分型及特异单倍体猪的培育和异种器官移植实验用动物的研究提供理论依据。
译 名：
Study on SLA Class Ⅰ-3 Gene for Inbreeding WZSP
作 者：
SUN Jun-li1,2,MU Yu-lian1,LIU Xiao-lin3,FENG Shu-tang1,WANG Su-rong4(1Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094;2Guangxi Institute of Animal Science,Nanning 530001;3 Northwest Agricultural & Forest University,Yangling 712100;4Chengde Vocational College,Chengde 067000)
关键词：
Inbreding Wuzhishan pig;Leucocyte antigen;SLA classical class-Ⅰ 3 gene;RT-PCR
摘 要：
【Objective】The purpose of this study is to elucidate the structure of SLA-3 alleles in inbred line of Wuzhishan pig(WZSP)population.【 Method 】 The partial exon1,completed exon2 and partial exon3 of SLA-3 loci using reverse transcription-polymerase chain reaction and sequencing-based method in 32 WZSPs were examined.According to the pedigree and amplification results,PCR products of 8 WZSPs were selected for cloning and sequencing.【Result】Nine different nucleotide sequences were obtained.After comparing DNA and protein sequences of the WZSPs SLA-3 alleles with published GenBank SLA sequences,these SLA-3 alleles found in WZSPs were all novel,but there were very few variation among them.Comparisions of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology.Meanwhile,construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that WZSPs population owns its unique genetics resource.【Conclusion】The alleles of SLA-3 in WZSPs group were successfully detected and analysed,which provided a firm basis on the genotype of SLA-3 and breeding specific haplotypes WZSPs.
