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当前位置: 首页 > 文章 > 壳寡糖诱导烟草抑制TMV增殖的研究 西北农林科技大学学报(自然科学版) 2006,34 (5) 88-92
Position: Home > Articles > Induced resistance to TMV multiplication in tobacco with Chito-oligosaccharides Journal of Northwest A & F University(Natural Science Edition) 2006,34 (5) 88-92

壳寡糖诱导烟草抑制TMV增殖的研究

作  者：
商文静;吴云锋;赵小明;杜昱光;商鸿生
单  位：
中国科学院大连化学物理研究所;西北农林科技大学植保学院
关键词：
烟草;壳寡糖;壳聚糖;系统诱导抗病性;烟草花叶病毒;病毒增殖
摘  要：
采用ELISA-DSM法和半叶枯斑法,研究了壳寡糖诱导对普通烟(Nicotiana tabacum)体内TMV 增殖的影响。结果表明,普通烟经50μg/mL壳寡糖溶液诱导处理后,其对TMV的侵染表达了高水平的系统抗病性,显症推迟4～7 d,平均严重度降低了82．9％。ELISA-DSM法检测结果表明,壳寡糖诱导处理的普通烟植株体内病毒浓度明显降低;在接种后10 d,接种叶片OD值仅为0．400,相当于同期不诱导对照的23．0%;新生叶片OD 值为0．190,相当于同期不诱导对照的38．7％。以发病普通烟叶片为接种体,利用半叶法接种心叶烟(Nicotiana glutinosa),结果表明,壳寡糖诱导处理大大减少了枯斑数目;以接种后10 d的壳寡糖处理叶片为接种体,平均半叶枯斑数为9．25,仅为不诱导对照的19．5%;以同期新生叶为接种体,平均半叶枯斑数为0．630,为不诱导对照的 25．0%。以上两种方法的检测结果均证明,壳寡糖诱导处理可以有效抑制普通烟体内的TMV增殖。通过对壳寡糖 (低分子质量)处理与壳聚糖(高分子质量)处理的比较发现,分子质量的差异不影响其对病毒增殖的抑制效果。
译  名：
Induced resistance to TMV multiplication in tobacco with Chito-oligosaccharides
作  者：
SHANG Wen-jing,WU Yun-feng,ZHAO Xiao-ming ,DU Yu-guang,SHANG Hong-sheng (College of Plant Protection,Northwest A & F University,Yangling Shaanxi 712100,China; Dalian Institute of Chemical Physics,Chinese Academy of Sciences, Dalian ,Liaoning 116023,China)
关键词：
tobacco;chito-oligosaccharide;chitosan; systemic induced resistance;TMV; virus multiplication
摘  要：
Chito-oligosaccharides are elicitors to systemic induced resistance to viral infection in tobacco (Nicotiana tabacum). The inhibitory effect to TMV multiplication in tobacco plants treated with chito-oligosaccharides was examined by ELISA-DSM and local lesion assays. The results showed high level resis- tance achieved with 4 -7 d delayed latent period and 82. 9% suppression of average severity in tobacco plants treated with a solution of chito-oligosaccharides at a concentration of 50 /μg/mL. In ELISA-DSM assay chito-oligosaccharides were shown to be able to inhibit multiplication of viruses in infected plants. The OD value in inoculated leaves (10 d after inoculation) from chito-oligosaccharides treated plants was 0.400,which was equal to 23. 0% of that from untreated control plants,and 0. 190 in fresh leaves from treated plants,or 38. 7% of that from untreated control plants on the same conditions. In local lesion assay the number of local lesions per half-leaf of N. glutinosa infected by TMV inoculum from detached leaves of tobacco treated with chito-oligosaccharides,was largely reduced. For example,in the assay with detached inoculated leaves (10 d after inoculation),the average number of local lesions was 9. 25 in chito-oligosaccharides treatment,or 19. 5% of that in untreated control,and in the assay with fresh leaves only 0. 63 local lesions produced in chito-oligosaccharides treatment,equal to 25. 0% of that in untreated control. The results obtained from two assays revealed that treatment with chito-oligosaccharides effectively inhibited TMV multiplication in tobacco. Besides,it was found that molecular weight of compounds did not influence the inhibitory effect contrast to treatments with chito-oligosaccharides (low molecular weight) or with chitosan (high molecular weight).

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