Study on Preparation of Biodiesel from Waste Cooking Oil of Hot Pot by Kete Immobilized Lipase

ZHENG Xu-xu1a,1b,ZHU Jun-ren1a,2,YIN Zhong-yi1a,1b,LI Qiang1a,ZHANG Yu-jing1a(1a.College of Environment and Bioengineering;1b.Engineering Research Centre for Waste Oil Recovery Technology and Equipment,Ministry of Education,Chongqing Technology and Business University,Chongqing 400067,China;2.Institute of Urban Construction and Environmental Engineering,Chongqing University,Chongqing 400045,China)

kete immobilized lipase;waste cooking oil;biodiesel;enzymatic

Using waste oil of Chongqing hotpot as raw material,methanol as acyl acceptor,kete immobilized lipase as catalyst,the preparation process of biodiesel from waste cooking oil of hot pot was studied by orthogonal test.The results showed that after pretreatment of the raw oil,when the molar ratio of methanol to oil was 4.0∶1.0,adding methanol for 3 times,the 20.0%(m/m) of lipase consumption,10%(m/m) of n-hexane amount,10%(m/m) of water consumption,water bath at 50 ℃,shaking at a speed of 100 r/min in shake flask system for 24 h,the bio-diesel yield would be 83.75%.When the molar ratio of methanol to oil was 3.0∶1.0,adding methanol 3 times,lipase dosage 25.0%(m/m),11%(m/m) of the amount of n-hexane,10%(m/m) of water consumption,water bath at 48 ℃,reacting in the column with a height to diameter ratio of 12.5∶1.0 at a flow rate of 0.6 mL/min in the fixed bed unit for 24 h,the yield of bio-diesel would be 80.95%.The physical and chemical indexes of the obtained biodiesel after vacuum distillation meet the standard of domestic 0# diesel oil,thus was replicable.