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新疆红果桑果实总生物碱的提取工艺研究

作  者:
菲鲁热·艾尼瓦尔;汗泥沙·肉孜;帕提古丽·马合木提
单  位:
新疆大学生命科学与技术学院
关键词:
红果桑果实;总生物碱;提取工艺
摘  要:
采用分光光度法,以盐酸小檗碱为标准品测定新疆红果桑中的总生物碱含量。在提取过程中通过单因素试验分析乙醇体积分数、料液比、提取时间及提取温度4个主要因素对提取率的影响。在单因素试验的基础上通过正交设计法进行试验,优化红果桑总生物碱提取工艺条件。结果表明:红果桑总生物碱的最佳提取工艺条件为乙醇体积分数90%、料液比1:15、提取温度70℃、提取时间6h。根据最佳提取工艺条件测得的红果桑的总生物碱提取率为2.5%。该实验结果可靠,方法简便,最佳条件适合批量生产中该药材的提取。
译  名:
Extraction of Total Alkaloids in Red Mulberry (Morus rubra L.) Fruits from Xinjiang
作  者:
FILORA Anwar,HANNISA Rozi,PTIGUL Mahmut(College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China)
关键词:
Morus rubra L.;alkaloids;berberine hydrochloride
摘  要:
Alkaloids in red mulberry(Morus rubra L.) were extracted with aqueous ethanol,and quantified using spectropho-tometry method and berberine hydrochloride as standard.Effects of ethanol concentration,solid-to-liquid ratio,extraction time and temperature on the alkaloids extraction ratio were studied and optimized by conducting orthogonal test.The optimum extracting conditions were that the red mulberry was extracted with 90% aqueous ethanol,and extracted at 70 ℃ for 6 h with the solid-to-liquid ratio at 1:15.Under such conditions,the extraction yield of alkaloids was 2.5%.
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