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当前位置: 首页 > 文章 > 钼对小鼠肾小管上皮细胞氧化损伤和骨架蛋白的影响 中国兽医科学 2016 (9) 1201-1206
Position: Home > Articles > Effect of molybdenum-induced toxicity on oxidative damage and cytoskeletal protein of mouse renal proximal tubular cells Chinese Veterinary Science 2016 (9) 1201-1206

钼对小鼠肾小管上皮细胞氧化损伤和骨架蛋白的影响

作  者：
杜伯强;杨自军;赵红先;张才;汪纪仓;孔涛;李光照;张可可
单  位：
河南科技大学动物科技学院环境与畜产品安全河南高校重点学科开放实验室
关键词：
钼;肾小管上皮细胞;细胞骨架;MTT;氧化损伤;免疫印记;实时荧光定量PCR
摘  要：
为研究钼(Mo)对小鼠肾小管上皮细胞的抗氧化功能及细胞骨架蛋白的影响,以小鼠原代肾小管上皮细胞为试验材料,通过向细胞培养基中添加0、0.1、0.2、0.4、0.8 mmol/L不同浓度的Mo并培养24 h,用MTT法测定Mo对肾小管上皮细胞活力的影响,比色法测定氧化损伤程度,实时荧光定量PCR和Westernblot对细胞骨架蛋白Vimentin、α-actin、β-actin的m RNA和蛋白的表达进行测定。结果显示,与对照组(Mo剂量0组)相比,试验组(Mo剂量0.1、0.2、0.4、0.8 mmol/L组)小鼠细胞内的SOD和T-AOC均有下降(P<0.01),而MDA升高(P<0.01),LDH含量随Mo剂量的增加呈先下降后上升趋势(P<0.01),CAT含量随Mo剂量的增加呈先上升后下降趋势(P<0.01);同时,与对照组相比,试验组Vimentin、α-actin、β-actin的mRNA和蛋白的表达均随Mo剂量的增加而下降(P<0.01或P<0.05)。结果表明,高于0.4 mmol/L浓度的Mo作用24 h后,小鼠肾小管上皮细胞抗氧化能力受到抑制并加剧了细胞的氧化损伤,同时还会抑制Vimentin、α-actin、β-actin的mRNA和蛋白的表达。
译  名：
Effect of molybdenum-induced toxicity on oxidative damage and cytoskeletal protein of mouse renal proximal tubular cells
作  者：
DU Bo-qiang;YANG Zi-jun;ZHAO Hong-xian;ZHANG Cai;WANG Ji-cang;KONG Tao;LI Guang-zhao;ZHANG Ke-ke;College of Animal Science and Technology,Henan University of Science and Technology/Henan Provincial Open Laboratory of Key Disciplines for Environmental and Animal Products Safety;
关键词：
molybdenum;;renal proximal tubular cells;;cytoskeleton;;MTT;;oxygen damage;;Western blotting;;real time quantitative PCR
摘  要：
This study aims to investigate the effect of high Mo on oxidative damage and cytoskeletal proteins in primary mouse renal proximal tubular cells(MRPTC).With different concentration of Na_2MoO_4,MRPTC were cultured for 24 h.Then,MTT method and kits methods were used to investigated cell vitality and oxidative damage,and q RT-PCR and Western-blot were used to detecte the changes of Vimentin,α-actin and β-actin mRNA and protein level.Results showed that after 24 h,SOD and T-AOC were decreased with increasing dose of Mo(P<0.01);MDA were increased with decreasing dose of Mo P<0.01;LDH was droped after the first rise with the dose increasing(P<0.01).CAT rose after the first drop with the dose increasing(P<0.01);α-actin,Vimentin,and β-actin mRNA and protein levels decreased with increasing the does of Mo(P<0.05 or P<0.01).In conclusion,high doses of Mo can cause significant oxidative damage and inhibit Vimentin,α-actin and β-actin expression in MRPTC.

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