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Position: Home > Articles > Research on method of extracting DNA from Apricot Kate Journal of Shandong Forestry Science and Technology 2004 (4) 3

两种方法提取凯特杏DNA的研究

作  者:
张玲;刘静;李继红;孙庆华;孙仲序
单  位:
山东农业大学;泰安市林科所;山东农业大学 271018
关键词:
凯特杏;DNA;电泳;分光光度计
摘  要:
采用CTAB法和SDS法 ,分别从杏的幼叶、嫩芽中提取DNA ,通过计算产率 ,测定OD2 60和OD2 80的比值认为 ,CTAB比SDS法提取的DNA数量和纯度都高 ,而且两种方法都表明幼嫩材料中提取的DNA数量比成熟叶片高出 2倍之多 ,纯度也较高。
译  名:
Research on method of extracting DNA from Apricot Kate
关键词:
Apricot; extracting DNA; electrophoresis; spectrophotometer
摘  要:
We have extracted DNA from young leaves, young bud and mature leaves by the way of CTAB and SDS. We calculate the production and measure OD260/OD280. The results show that quantity and purification degree of extracted DNA by the method CTAB is higher than that by the method SDS. We also find that quantity of extracted DNA from young material is two times higher than that from mature leaves, purification degree of DNA from mature leaves is also lower than that of young material.

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