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当前位置: 首页 > 文章 > 猪LIAS基因微环表达载体的构建 畜牧兽医学报 2014 (3) 380-384
Position: Home > Articles > Construction of Minicircle Vector Carrying Porcine LIAS Gene Chinese Journal of Animal and Veterinary Sciences 2014 (3) 380-384

猪LIAS基因微环表达载体的构建

作  者：
高会贞;王晓婷;郭豫杰;韩立强;鲁维飞;杨国宇
单  位：
河南农业大学
关键词：
猪;硫辛酸合成酶;基因;微环表达载体;HeLa细胞
摘  要：
为了构建猪LIAS基因长期高效的表达载体,本研究通过RT-PCR方法从猪肌肉组织中克隆了LIAS基因片段,并将该基因片段与微环表达载体minicircle进行连接,构建成微环载体minicircle-LIAS,然后转染HeLa细胞,检测其在HeLa细胞中绿色荧光蛋白的持续表达时间。结果表明,本研究克隆了猪LIAS基因,该基因长度为1 119bp,并将该基因成功构建到了微环表达载体minicircle上;转染HeLa细胞对绿色荧光蛋白持续表达时间的观察显示,构建的微环表达载体minicircle-LIAS中绿色荧光蛋白的持续表达时间明显高于常用的pEGFP-N1质粒,为进一步阐明LIAS合成的生化过程及功能调控提供了新思路和试验材料。
译  名：
Construction of Minicircle Vector Carrying Porcine LIAS Gene
作  者：
GAO Hui-zhen;WANG Xiao-ting;GUO Yu-jie;HAN Li-qiang;LU Wei-fei;YANG Guo-yu;Key Laboratory of Animal Biochemisty and Nutrition,Ministry of Agriculture,Henan Agricultural University;
关键词：
porcine;;lipoic acid synthase;;gene;;minicircle vector;;HeLa cells
摘  要：
In order to construct a long-term and high-level transgene expression recombinant vector,LIASgene was cloned from the muscle of porcine by RT-PCR and inserted into minicircle plasmid.The minicircle-LIAS vector was transfected into HeLa cells to investigate the duration of GFP expression.The results showed that the LIASgene which was 1 119bp length was completely cloned and inserted into minicircle plasmid.The vector was transfected into HeLa cells and the duration of GFP expression of recombinant minicircle-LIAS vector was obviously higher than that of pEGFP-N1plasmid.This result provides a new thought and experimental material for the process of LIAS biological synthesis and function regulation.

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