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当前位置: 首页 > 文章 > 侵染番茄的黄瓜花叶病毒、烟草花叶病毒和马铃薯S病毒CP基因克隆及序列分析 北方园艺 2017 (22) 12-18
Position: Home > Articles > Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS FromLycopersicon esculentum Mill. Plant Northern Horticulture 2017 (22) 12-18

侵染番茄的黄瓜花叶病毒、烟草花叶病毒和马铃薯S病毒CP基因克隆及序列分析

作  者：
于沛侠;赵慧琪;刘勇;王德富;牛颜冰
单  位：
山西农业大学生命科学学院
关键词：
番茄;复合侵染;烟草花叶病毒;黄瓜花叶病毒;马铃薯S病毒
摘  要：
以山西省晋中地区表现褪绿黄化、卷曲、叶脉变黑症状的番茄植株叶片为试材,采用双链RNA(double-stranded RNA,dsRNA)技术和非序列依赖PCR扩增(Sequence-independent amplification,SIA)方法,对侵染番茄病样的病毒病原进行检测,以期为山西省番茄病毒病的防治提供参考依据。结果表明:该病样由黄瓜花叶病毒(CMV)、烟草花叶病毒(TMV)和马铃薯S病毒(PVS)3种病毒复合侵染,将其分别命名为CMV-SXFQ、TMV-FQ和PVS-SXFQ。为明确CMV-SXFQ、TMV-FQ和PVS-SXFQ的分类地位,进一步克隆到了这3个病毒的CP基因序列并进行相似性比较,发现CMV-SXFQ与CMV各亚组代表株系相似性为74.9%~98.8%,氨基酸序列的相似性为82.5%~99.5%;TMVFQ与TMV代表株系相似性为74.4%~99.8%,氨基酸序列的相似性为83.6%~100.0%;PVS-SXFQ与PVS代表株系相似性为93.2%~99.3%,氨基酸序列的相似性为92.4%~100.0%;系统进化分析表明:CMV-SXFQ分离物与亚组ⅠB的CH、HLJ、XJ2、Am、As株系亲缘关系最近;TMV-FQ分离物与IM、Jimo、SXFQ株系亲缘关系最近;PVSSXFQ分离物与Cm、St株系亲缘关系最近。
译  名：
Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS FromLycopersicon esculentum Mill. Plant
作  者：
YU Peixia;ZHAO Huiqi;LIU Yong;WANG Defu;NIU Yanbing;College of Life Sciences,Shanxi Agricultural University;Hunan Plant Protection Institute,Hunan Academy of Agricultural Science;
单  位：
College of Life Sciences,Shanxi Agricultural University%Hunan Plant Protection Institute,Hunan Academy of Agricultural Science
关键词：
Lycopersicon esculentum Mill.;;mixed infection;;tobacco mosaic virus;;cucumber mosaic virus;;potato virus S
摘  要：
The leaves of tomato(Lycopersicon esculentum)from Jinzhong region of Shanxi Province shown chlorotic yellows,curly,black leaf pathogen were used as materials,the technology of doublestranded RNA(dsRNA)and sequence-independent amplification(SIA)was used to identify the virus pathogen that induced tomato,in order to provide reference for the prevention and control of virus disease infecting tomato in Shanxi Province.The results showed that samples of plants were infected with tobacco mosaic virus(TMV),cucumber mosaic virus(CMV)and potato virus S(PVS)respectively and named CMV-SXFQ,TMV-FQ and PVS-SXFQ.To further characterize the isolate of CMV-SXFQ,TMV-FQ and PVS-SXFQ,the coat protain(CP)of CMV-SXFQ,TMV-FQ and PVSSXFQ were cloned and analyzed.Sequence alignments showed that the nucleotide homology similarity between CMV-SXFQ and CMV subgroup virus were 74.9%-98.8%,and the amino acide sequence similarity were 82.5%-99.5%.The nucleotide homology similartity between TMV-FQ and TMV subgroup virus was 74.4%-99.5%,and the amino acide sequence similarity were 83.6%-100.0%.The nucleotide homology similartity between PVS-SXFQ and PVS subgroup virus were 93.3%-99.3%,and the amino acide sequence similartity were 92.4%-100.0%.Phylogenetic analysis suggested that CMV-SXFQ was the same as CH,HLJ,XJ2,Am and As,which belonged to the CMV subgroup I B;TMV-FQ was closely related to IM,Jimo and SXFQ;PVS-SXFQ was closely related to Cm,St.

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