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Position: Home > Articles > Cloning and Functional Analysis of PdSIKI from Populus deltoides × Populus nigra Journal of Anhui Agricultural Sciences 2016 (7) 98-102

欧美杨PdSIKI基因的克隆与功能分析

作  者:
杨李娇;郭鹏;许涵杰;郭鹏
单  位:
大连民族大学
关键词:
欧美杨;PdSIKI基因;克隆;表达;干旱
摘  要:
[目的]筛选抗旱节水欧美杨品种。[方法]以欧美杨为材料,通过RT-PCR方法从欧美杨叶片中克隆到抗旱PdSIKI基因,将其转入拟南芥中,研究转PdSIKI基因拟南芥的表型性状和生理指标。[结果]PdSIKI基因开放阅读框为2 442 bp,编码813个氨基酸。蛋白质分析表明PdSIKI蛋白含有典型的类受体蛋白激酶高度保守的丝氨酸/苏氨酸结构域和跨膜区域。荧光定量PCR分析表明该基因在顶端叶中表达量最高,功能叶、根和茎中则无表达。逆境胁迫分析表明该基因受Na Cl、ABA和干旱诱导表达明显。与野生型相比,干旱处理下转PdSIKI基因拟南芥表现出更强的生长状态,且叶绿素含量降低,丙二醛含量升高。[结论]超表达PdSIKI基因提高了拟南芥抗干旱能力。
译  名:
Cloning and Functional Analysis of PdSIKI from Populus deltoides × Populus nigra
作  者:
YANG Li-jiao;GUO Peng;XU Han-jie;GUO Peng;Dalian Nationalities University;
关键词:
Populus deltoides × Populus nigra;;PdSIKI;;Cloning;;Expression;;Drought stress
摘  要:
[Objective] The aim was to screen out drought resistance and water-saving Populus deltoides × Populus nigra varieties. [Method]With Populus deltoides × Populus nigra as materials,drought resistance PdSIKI gene was cloned from Populus deltoides × Populus nigra leaves with RT-PCR method then transferred into Arabidopsis thaliana,the phenotypic traits and physiological indicators were studied.[Result]The c DNA of PdSIKI was 2 442 bp and contained a single open reading frame of 813 amino acid residues. Protein sequence analysis showed the PdSIKI has a serine / threonine protein kinase domain and transmembrane. Real time-PCR indicated that the m RNA accumulation of PdSIKI was strongly expressed in immature leaves,but not in functional leaves,roots and stem and induced by salt stress,ABA and drought. Additionally,compared with the control,transgenic PdSIKI arabidopsis performed stronger growth status. Chlorophyll content decreased and MDA content increased in drought stress.[Conclusion] PdSIKI gene can improve drought resistance ability of Arabidopsis thaliana.

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