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Position: Home > Articles > Establishment of Immunohistochemical Localization of Rabies Virus Journal of Jilin Agricultural University 2009,31 (6) 87-90

狂犬病病毒免疫组织化学定位方法的建立

作  者:
王泽;夏志平;江禹;连海;毛文俊;钱爱东
单  位:
吉林农业大学动物科技学院;成都军区空军食品基地;军事医学科学院军事兽医研究所
关键词:
免疫组织化学;狂犬病病毒;检测方法
摘  要:
应用抗狂犬病病毒的特异性抗体及免疫组织化学方法检测接种了狂犬病病毒SRV-9毒株感染的小鼠脑组织,并对狂犬病病毒抗原进行了组织定位分析。结果表明:在小鼠的小脑分子层蒲肯野氏细胞胞浆出现明显的局灶型阳性颗粒。说明免疫组织化学方法检测狂犬病病毒敏感度高、直观,可作为诊断狂犬病病毒的辅助性方法,对研究狂犬病病毒在脑组织神经细胞及其他组织器官内的分布具有一定的意义。
译  名:
Establishment of Immunohistochemical Localization of Rabies Virus
作  者:
WANG Ze1,2,XIA Zhi-ping1,JIANG Yu1,LIAN Hai1,MAO Wen-jun3,QIAN Ai-dong21.Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130062,China;2.College of Animal Science and Technology,Jilin Agricultural University, Changchun 130118,China;3.Food Basis for Chengdu Airforce,Chengdu 610000,China
关键词:
immunohistochemistry;rabies virus;detection method
摘  要:
Rabies virus antigen in suckling brain tissues of mice infected with rabies virus by the specific antibody and immunohistochemistry technique was detected and the localization of rabies virus antigen in the brain tissues was analyed.The results showed that there were positive grains in the molecular layer of cerebellum.We can see that immunohistochemistry technique with labeled specific antibody was sensitive in detecting rabies virus and it can be an adjuvanticity method for detecting rabies virus and has certain significance for studying the distribution of rabies virus in mice brain tissues and determination of nervous cell containing rabies virus as well.

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