当前位置: 首页 > 文章 > 小鼠胚胎分割方法及同卵双生试验 西北农林科技大学学报(自然科学版) 1987 (2) 10-16
Position: Home > Articles > PROCEDURES OF BISECTING EMBRYOS AND PRODUCTION OF MONOZYGOTIC TWINS IN MOUSE Journal of Northwest A & F University(Natural Science Edition) 1987 (2) 10-16

小鼠胚胎分割方法及同卵双生试验

作  者:
张涌;钱菊汾;王建辰
关键词:
胚胎分割;半胚;同卵双生;小鼠
摘  要:
选择小鼠晚期桑椹胚和早期囊胚,采用下列方法进行分割:A、显微手术刀直接分割;B、酶消化透明带后分割;C、显微玻璃针去带分割;D 酶——机械去带分割。共分割277枚胚胎,分割成功264枚(95.3%)。将其中480枚半胚作体外培养,A,B,C,D 各组半胚发育到囊胚的发育率分别为65.7%(92/140)、54.2%(65/120)、63(63/100)、64.2%(77/120)。将用 A法分割的48枚半胚移植于12只受体鼠,结果有4只怀孕,共产11只半胚鼠,其中4对有同卵双生仔鼠。
译  名:
PROCEDURES OF BISECTING EMBRYOS AND PRODUCTION OF MONOZYGOTIC TWINS IN MOUSE
作  者:
Zhang Yong Qian Jufen Wang Jianchen (Department of Veterinary Medicine,Northwestern Agricultural University)
关键词:
bisection of embryos;;half embryos;;monozygotic twins;;mouse
摘  要:
Late morulae and early blastocysts were selected to carry out the embryos bisection with the following procedures:A.by a microblade directly;B.by a microblade after removal of the zonae pellucide by digestion with pronase; C.by a fine glass needle after mechnical removal of the zonae pellucide;D. by a microblade after enzymic and mechnical removal of the zonae pellucide. Of 277 embryos,264(95.3%)were successfully bisected by the above pro- cedures.480 thalf embryos were cnltured in vitro.The development rate of them to blastocysts in A,B,C,and D groups was 65.7%(92/140);54.2% (65/120);63%(63/100)and 64.2%(77/120)respectively.48 half embryos bisected by the microblade were transfered to 12 pseudopregnant recipients,of which 4 became pregnant.11 live young were obtained at delivery with 4 pairs of monozygotic twins included.

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