中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 利用AFLP鉴定沙田柚新种质南方农业学报 2012,43 (11) 28-31

Position: Home > Articles > Identification of new Shatian pumelo(Citrus grandis L.) germplasm by AFLP Journal of Southern Agriculture 2012,43 (11) 28-31

利用AFLP鉴定沙田柚新种质

作者：

肖远辉;麦适秋;傅翠娜;区善汉;梅正敏;廖奎富;徐粹明

单位：

广西柑桔研究所

关键词：

沙田柚;桂柚1号;早熟沙田柚;遗传鉴定;AFLP

摘要：

【目的】鉴定沙田柚新种质的遗传分类,为其利用提供参考。【方法】选用64对AFLP引物组合,对12份柚类种质资源进行遗传多样性分析及分类研究。【结果】从64对引物中筛选出12对AFLP引物组合,在12份柚类材料中扩增出399条清晰条带,每对引物扩增条带数为25～49条,平均每对引物扩增条带33.25条;多态性条带91条,多态性比例为22.8%。供试材料相似系数在0.752～0.956,AFLP鉴定结果显示桂柚1号与沙田柚相似系数为0.956,早熟沙田柚与沙田柚相似系数为0.920;早熟酸柚和福建酸柚两个品种与其他柚类亲缘关系较远;以0.830为相似系数的阈值,可以将12份柚类材料分为5类。【结论】AFLP分子标记技术能将12份柚类材料区分开;早熟沙田柚与桂柚1号是不同于沙田柚的变异新种质,可以直接作为品种资源加以利用。

译名：

Identification of new Shatian pumelo(Citrus grandis L.) germplasm by AFLP

作者：

XIAO Yuan-hui,MAI Shi-qiu,FU Cui-na,OU Shan-han*,MEI Zheng-min,LIAO Kui-fu,XU Cui-ming(Guangxi Citrus Institute,Guilin,Guangxi 541004,China)

关键词：

Shatian pomelo;Guiyou 1;early-ripening Shatian pomelo;genetic identification;AFLP

摘要：

Objective The genetic diversities of Shatian pummelo(Citrus grandis L.) were identified and classified in order to provide references for future usage.Method Out of the 64 selected pairs of AFLP primer combination,12 pummelos materials were carefully classified and analyzed.Result From the 64 primers,12 pairs of AFLP primer combinations were screened out.The 12 tested materials were amplified with 399 clear bands,in which each pair of primers amplified 25 to 49 bands;the average number of amplified bands for each pair of primers was 33.25.The polymorphic band number was 91,and the polymorphism ratio was 22.8%.The similarity coefficients of the tested materials were between 0.752 and 0.956.The AFLP identification results showed that similarity coefficient of Guiyou 1 with Shatian pomelo was 0.956,and the similarity coefficients of early-ripening Shatian pomelo and Shatian pomelo was 0.920.The two pomelo varieties,early-ripening sour pomelo and Fujian sour pomelo,were distantly related to other pomelo varieties.Using the similarity coefficient threshold of 0.83,the 12 tested pomelo materials were divided into 5 groups.Conclusion The AFLP molecular marker technology could successfully separate the 12 tested pomelo materials.Early-ripening Shatian pomelo and Guiyou 1 were different from the other pomelo variations,and could be directly exploited as variety resources.

相似文章

多效唑对沙田柚采后伤果的诱导抗病效应 [张镜, 刁俊明, 黄思梅, 刁树平] 食品科学 2010,31 (20) 453-458
沙田柚果园施用猪沼液替代化肥初探 [张明来] 东南园艺 2021 (4) 45-51
不同处理对桂柚1号与沙田柚保果的影响南方园艺 2021 (2) 1-3
衰老沙田柚低产果园土壤营养状况分析 [张社南, 梅正敏, 贺申魁, 袁洁, 区善汉, 刘冰浩, 欧阳培, 肖远辉, 莫健生] 南方园艺 2021 (3) 1-4
利用LC-MS技术解析沙田柚、琯溪蜜柚种子代谢物的差异 [崔学宇, 彭玉娇, 陈众峰, 李静, 邵元元, 侯彦林, 贾书刚] 种子 2021 (2) 1-6
沙田柚保健果醋酿造工艺研究 [杜冰, 温升南, 李燕杰, 杨公明] 食品科学 2008,29 (12) 255-258
昭平柚农信心百倍抓夏管 [] 中国果业信息 2007 (005) 35
昭平柚农齐抓柚树授粉 [蒙安德] 中国果业信息 2006,023 (004) 41
1000μL·L^-1乙烯利处理可显著促进沙田柚采后果皮褪绿 [杨朴丽] 中国果业信息 2013 (008) 65
沙田柚矮化病的调查和嫁接传染性的测定 [邱柱石, 黄美玲] 广西植保 1996 (2) 9-16

导出引用

计量

文章访问数: 16

HTML全文浏览量: 0

PDF下载量: 0

利用AFLP鉴定沙田柚新种质

相似文章

所属期刊

南方农业学报

推荐期刊

现代农业科技

中国果业信息