作 者：
王聪;杨恒山;刘艳华;董永义;马玉露;贾俊英;包金花;郑毅
单 位：
内蒙古民族大学农学院;内蒙古赤峰市农牧业水利局农业技术推广站
关键词：
外源壳聚糖;NaCl胁迫;叶绿体;AsA-GSH循环
摘 要：
为探讨壳聚糖对NaCl胁迫下光合作用的调节机制,采用盆栽试验方法研究了外源壳聚糖对NaCl胁迫下菜用大豆[Glycine max(L.)Merr.]叶绿体内AsA-GSH循环的影响。选用菜用大豆主栽品种绿领特早为试验材料,设4个处理:1叶面喷洒清水,根部浇灌营养液(对照);2叶面喷洒壳聚糖溶液,根部浇灌营养液;3叶面喷洒清水,根部浇灌溶有NaCl的营养液;4叶面喷洒壳聚糖溶液,根部浇灌溶有NaCl的营养液。结果表明,外源壳聚糖阻止了NaCl胁迫下菜用大豆干质量的下降,显著降低了叶绿体H_2O_2含量,显著提高了抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPX)活性以及还原型谷胱甘肽(GSH)含量和胁迫中期还原型抗坏血酸(AsA)含量。外源壳聚糖对NaCl胁迫下菜用大豆叶绿体内AsA-GSH循环产生了显著促进作用,使AsA-GSH循环保持较强的活性氧清除能力,这可能是其维持较强光合能力,进而减缓干质量下降的重要原因之一。
译 名：
Influences of exogenous chitosan on AsA-GSH cycle in vegetable soybean chloroplast under NaCl stress
作 者：
WANG Cong;YANG Heng-shan;LIU Yan-hua;DONG Yong-yi;MA Yu-lu;JIA Jun-ying;BAO Jin-hua;ZHENG Yi;College of Agriculture,Inner Mongolia University for Nationalities;Agricultural Technology Extension Station,Chifeng City,Inner Mongolia Water Conservancy Bureau of Agriculture and Animal Husbandry;
关键词：
exogenous chitosan;;NaCl stress;;chloroplast;;AsA-GSH cycle
摘 要：
In order to investigate the mechanism of photosynthesis regulation by chitosan under NaCl stress,the effects of exogenous chitosan on AsA-GSH cycle in the chloroplasts of NaCl-stressed vegetable soybean [Glycine max( L.)Merr. ] was studied. Four treatments were set for soybean Lutingtezao:( 1) foliar water spraying and root watering with mutrient solution( control);( 2) foliar chitosan solution spraying and root watering with nutrient solution;( 3) foliar water spraying and root watering with NaCl solution; and( 4) foliar chitosan solution spraying and root watering with NaCl solution. The results showed that exogenous chitosan prevented vegetable soybean dry mass from declining,decreased chloroplast H_2O_2 content and improved the contents of ascorbate peroxidase( APX), monodehydroascorbate reductase( MDHAR),dehydro ascorbic acid reductase( DHAR),glutathione reductase( GR) and glutathione peroxidase( GPX) activities and dehydroascorbic glutathione( GSH), as well as dehydroascorbic acid( AsA) during the mid-term of NaCl stress,suggesting that exogenous chitosan facilitated the AsAGSH cycle of vegetable soybean chloroplast,so that the AsAGSH cycle maintained strong activity of reactive oxygen scavenging,which may be one of the key reasons to keep strong photosynthetic capacity to prevent dry mass from declining.
