作 者：
李艳;王磊;蔡汝健;蒋智勇;勾红潮;楚品品;宋帅;李春玲
关键词：
抗体芯片;猪葡萄球菌;炎性细胞因子;分子标记物
摘 要：
为分析猪葡萄球菌感染BALB/c小鼠不同时间点血清中炎性细胞因子表达的变化,收集猪葡萄球菌感染BALB/c小鼠不同感染时段(24、48 h)的血清,采用细胞因子抗体芯片技术检测32种细胞因子水平.结果显示,猪葡萄球菌感染小鼠后血清细胞因子表达水平发生了明显变化:与对照相比,感染后24 h,血清细胞因子中有5种细胞因子表达出现了显著性变化,其中表达升高2倍以上的细胞因子有4种(G-CSF、IL-6、KC、MCP-5);感染后48 h,血清细胞因子中共有4种细胞因子表达出现了显著性变化,其中表达升高2倍以上的细胞因子有3种(G-CSF、KC、MCP-5);感染24 h和48 h后IL-12的表达均显著低于对照.炎性细胞因子在猪葡萄球菌感染中发挥重要作用,利用细胞因子抗体芯片从血清中筛选猪葡萄球菌相关蛋白分子标记物是可行的.
作 者：
LI Yan;WANG Lei;CAI Ru-jian;JIANG Zhi-yong;GOU Hong-chao;CHU Pin-pin;SONG Shuai;LI Chun-ling;Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Key Laboratory of Livestock Disease Prevention of Guangdong Province , Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture;
关键词：
antibody chip;;Staphylococcus hyicus;;inflammatory cytokine;;molecular marker sequencing
摘 要：
In order to analyze the expression of inflammatory cytokines in serum of BALB/c mice infected with Staphylococcus hyicus at different time point, the serum of the BALB/c mice infected with staphylococcus hyicus were collected at both 24 h and 48 h post infection, and the levels of 32 cytokines were detected by cytokine antibody chip technology. The results showed that there was a significant change in the level of cytokine from serum in mice infected with Staphylococcus hyicus. Compared with the control group, there were 5 cytokines significantly changed 24 h post infection. Among them, 4 cytokines(G-CSF, IL-6, KC, MCP-5) were expressed 2 times greater than controls. After infected 48 h, 4 cytokines significantly changed, and 3 of them, including G-CSF, KC, MCP-5, were expressed 2 times greater than the control levels. The expression level of IL-12 were significantly lower than the control levels at both 24 h and 48 h after infection. The inflammatory cytokines play an important role in the process of S. hyicus infection. It is feasible to screen staphylococcus hyicus related protein biomarkers from serum by applying cytokine antibody array.
