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Position: Home > Articles > Isolation of Riemerella anatipestifer and Identification of 16 S rRNA Progress in Veterinary Medicine 2010,31 (6) 77-80

鸭疫里氏杆菌的分离与16 S rRNA的鉴定

作  者:
冯金牛;阮二垒;杨丽云;黎丁滔;陈瑞爱;王林川
单  位:
广东省大华农动物保健品股份有限公司;华南农业大学兽医学院
关键词:
鸭疫里氏杆菌;生化鉴定;16SrRNA;PCR
摘  要:
从疑似患有鸭疫里氏杆菌病的病死鸭群中采取的肝脏、心脏、脑等病料中分离病原,进行生化鉴定,自6只病死鸭的12份病料中分离鉴定出6株鸭疫里氏杆菌。根据鸭疫里氏杆菌16 S rRNA基因的保守序列设计特异引物,对6株鸭疫里氏杆菌进行PCR扩增,均能扩增出680 bp的特异条带;从凝胶中回收DNA目的条带并测序,测序结果与GenBank中鸭疫里氏杆菌相应序列相似率达到99.99%。
译  名:
Isolation of Riemerella anatipestifer and Identification of 16 S rRNA
作  者:
FENG Jin-niu1,RUAN Er-lei1,YANG Li-yun1,LI Ding-tao1,CHEN Ri-ai2,WANG Lin-chuan1,2(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China;2.Guanggdong Dahuanong Animal Health Products Co.Ltd,Xinxing,Guangdong,527400,China)
关键词:
Riemerella anatipestifer;biochemical identification;16 S rRNA;PCR
摘  要:
Fecal samples were collected from sick and dead ducks likely caused by Riemerella anatipestifer in a duck farm.The pathogens were isolated and biochemically identificated,6 strains of bacteria were isolated from 12 fecal samples.According to the highly conservative nature of bacterial 16 S rRNA gene,a set of primers with broad range for Riemerella anatipestifer were designed,and 6 strains of bacteria were detected by PCR and compared with 16 S rRNA sequence of Riemerella anatipestifer from GenBank.The resembling rate can exceed 99.99%.

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