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当前位置: 首页 > 文章 > TCM199对陇东绒山羊细管精液的冷冻效果 畜牧兽医杂志 2012,31 (4) 34-36+38
Position: Home > Articles > Effect of TCM-199 Supplementation on Longdong Cashmere Goat Semen Diluents on Plastic Straw Frozen Semen Quality Journal of Animal Science and Veterinary Medicine 2012,31 (4) 34-36+38

TCM199对陇东绒山羊细管精液的冷冻效果

作  者：
王珂;贺洞杰;郭海龙;吕永锋;李振明;于轩
单  位：
甘肃省畜牧兽医研究所;甘肃宁县兴旺牧业公司;甘肃省畜牧技术推广总站;兰州大学生命科学院
关键词：
陇东绒山羊;TCM199;细管冻精
摘  要：
目的研究陇东绒山羊细管精液冷冻时,通过在葡萄糖-柠檬酸盐稀释液中添加TCM199,观察其对陇东绒山羊细管冷冻精冷冻效果的影响。方法选用4个试验组和对照组进行对比试验,试验组是在稀释液基础上分别添加TCM199标准液,含量为0.5%、1%、3%、5%(V/V)。结果表明:添加1%的TCM199标准液的试验组(B)效果较好,其冷冻解冻后精子活力高于对照组和其它试验组,但差异不显著(P>0.05),质膜完整率试验组和对照组相比差异不显著(P>0.05),在精子穿卵活力试验中(SPA),试验组和对照组相比差异不显著(P>0.05)。但在细管解冻后不同时间段活力测定试验中,解冻后5h、8h、12h,试验组(B)和对照组活力差异显著(P<0.05),且随着时间延长呈下降趋势。因此制作陇东绒山羊精液细管冷冻时,添加1%TCM199标准液具有比较好的效果。
译  名：
Effect of TCM-199 Supplementation on Longdong Cashmere Goat Semen Diluents on Plastic Straw Frozen Semen Quality
作  者：
WANG Ke1,HE Dong-jie2,GUO Hai-long1,LV Yong-feng1,LI Zhen-ming3,YU Xuan4,(1.Institute of Veterinary Medicine and Animal Husbandry of Gansu Province,Ping liang Gansu 744000,China; 2.Life Science College of Lanzhou University;3.Ningxian County Thriving Animal Husbandry Company of Gansu Province; 4.General Station of Gansu Animal Husbandry Management)
关键词：
Longdong cashmere goat;TCM-199;plastic straw frozen semen
摘  要：
The effect of TCM-199 supplementation on the Longdong cashmere goat frozen-thawed semen was studied through supplementation of TCM-199 supplementation in the glucose-citrate,a goat semen diluents.Four test groups,which were formulated on the varying basis diluents,doses of 0.5%,1%,3% and 5%(V/V)TCM-199,respectively,and control group were selected.The results showed that the test group B diluents was the best one among the diluents tested groups and its semen viability,plasma membrane integrity after-thawing and SPA were all higher than other three test groups and control group and there's no obvious statistic difference among them(P>0.05).However,semen viability of the test group B was significantly higher than control group diluents after-thawing according to different period(P<0.05).The results revealed a decreasing tendency along with the time.Therefore,the test group B was adaptive for making plastic straw frozen semen of Longdong cashmere goat.

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