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Position: Home > Articles > A Study on Dihaploid Induction of Potato Acta Agriculturae Boreali-Sinica 1999,14 (1) 35-39

马铃薯双单倍体诱导研究

作  者:
庞万福;屈冬玉;纪颖彪;姜淑荣
单  位:
中国农科院蔬菜花卉研究所;廊坊市农林科学院
关键词:
马铃薯;双单倍体;孤雌生殖;标记基因
摘  要:
通过 4x-2x杂交测验,从Solanum phureja后代中筛选出 9份诱导双单倍体频率和花粉育性高的授粉者,它们的平均诱导率为33株双单倍体/100个浆果,并具有显性纯合的胚斑标记基因(BB)和紫色胚轴标记基因(PP),可用来有效识别双单倍体。不同的四倍体母本产生双单倍体的频率不同,中薯2号产生频率最高,100个果中可产生34株双单倍体,母本基因型对双单倍体的产生有很大的影响。
译  名:
A Study on Dihaploid Induction of Potato
作  者:
Pang Wanfu Qu Dongyu Ji Yingbiao Jiang Shurong(1 Langfang Academy of Agriculrural and Forestry Sciences,Langfang 065000) (2 Institute of Vegetable and Floriculture,Chinese Academy of Agricultural Sciences,Beijing 100081)
关键词:
Potato; Dihaploid; Parthenogenesis; Marker gene
摘  要:
Through 4x-2x cross,9 lines from Solatium phureja were selected as pollinators. These pollinators had several advantages over the controls (IVP35 and IVP48) , including high frequency of dihaploid induction and pollen fertility. Their average frequency of induction was 33.0 dihap-loids/100 fruits. They also possessed a dominant embryo spot marker (BB) and a dominant seed-ing marker (PP) for purple hypocotyl. Both markers are homozygous and can be effcetively used to inentify dihaploids. There were differences in the dihaploid frequency between the maternal parents. Zhongshu 2 had the highest frequency (34.0 dihaploid/100 fruits) among three varicties. But the effects of environments on pseudogamous dihapoid production can never be neglected.

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