Position: Home > Articles > PRELIMINARY STUDY OF SOLID PHASE MICRO-EXTRACTION(SPME)AS A METHOD FOR DETECTING INSECT INFESTATION IN WHEAT GRAIN
Grain Storage
2014
(6)
12-19
顶空固相微萃取法(SPME)检测小麦受害虫感染情况初步研究
作 者：
Wathukan Laopongist;George Srzednicki;John Craske;方茜
单 位：
澳大利亚悉尼新南威尔士大学化学工程学院
关键词：
害虫感染;顶空固相微萃取法(SPME);昆虫信息素;指示性化合物;灵敏度
摘 要：
研究了一种新的用于检测储藏小麦害虫感染的方法——顶空固相微萃取法(SPME),实验结果表明:利用SPME法所提取的几种常见储粮害虫(谷蠹Rhyzopertha dominica、赤拟谷盗Tribolium castaneum、扁谷盗Cryptolestes ferrugineus与锈赤扁谷盗Cryptolestes pusillus)所释放的挥发性信息素可以作为粮食受害虫感染的检测指标,同时由于它们所释放的信息素量不同(谷蠹最高,其次分别是赤拟谷盗﹑扁谷盗和锈赤扁谷盗),这也直接导致了SPME法检测灵敏度的不同,实验结果表明:在1kg的储藏小麦中能够成功地检测出1头赤拟谷盗和1头谷蠹,而扁谷盗和锈赤扁谷盗则需要更多的数量才能够被检测出来。此外,提取时间与温度也会影响SPME的灵敏度,实验证明:较长的提取时间与较高的实验温度都会增加信息素的产量,通过多次验证最终认定最佳提取条件为:温度50℃,时间4h。总之,将SPME法应用到粮食仓储行业中用于检测粮食受害虫感染的程度虽已初见成果,但还需要进一步地深入研究,使用动态顶空进样法或许可以提高SPME技术的灵敏性。
译 名：
PRELIMINARY STUDY OF SOLID PHASE MICRO-EXTRACTION(SPME)AS A METHOD FOR DETECTING INSECT INFESTATION IN WHEAT GRAIN
作 者：
Wathukan Laopongsit;George Srzednicki;John Craske;School of Chemical Engineering,The University of New South Wales;
关键词：
insect infestation,solid phase microextraction(SPME),insect pheromones,indicator compounds,sensitivity
摘 要：
In this study,new method was investigated using solid phase microextraction(SPME)for detection of insect infestation in stored wheat.The results show that volatile pheromones isolated from a number of common stored grain insects,can be used to indicate infestation.The amounts of pheromone released varied between species.Rhyzopertha dominica(F.)was the highest volatile pheromone producer among the species investigated,then it was followed by Tribolium castaneum(Herbst),Cryptolestes ferrugineus(Stephens)and Cryptolestes pusillus(Schonherr),respectively.This resulted in different sensitivity when SPME was used for detection.A single specimen of T.castaneum and R.dominica could be successfully detected in 1kg of wheat,whereas more number of C.ferrugineus and C.pusillus beetles were required for detection in the same quantity of grain.Extraction period and temperature affected the sensitivity of the SPME method.Longer exposure time and higher temperature allowed more analytes to be collected.Consequently,extraction for 4hat 50 Cappeared to be the optimum condition for extracting pheromones produced by the beetles covered by this study.The method was further adjusted to be more practical and this resulted in changing the sensitivity.Detection of a single R.dominica could still be successfully achieved,even though the time and the temperature were reduced,whereas for the remaining species,the minimum number of insects required had to be increased by about 40e60%in order to detect volatile pheromone in headspace.In conclusion,the SPME technique in detection of infestation is still required further development to be applied to the grain industry.Dynamic headspace may help improve the sensitivity of technique.
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