作 者：
刘志刚;宋杰益;彭卫东;崔晓民;张以耕;涂卫国;余金星;周江东
单 位：
江西医学院寄生虫学教研室;江西奉新县卫生防疫站
摘 要：
本文首次比较了经中华硬蜱(Ixodes sinensis)叮咬三次后再经二棘血蜱(Haemaphysalisbispinosa)叮咬的家兔与仅经二棘血蜱叮咬的家兔的交叉免疫抗性。二棘血蜱叮咬被中华硬蜱致敏的家兔时,吸血增重为:143.12±32.67mg,但二棘血蜱在正常家兔体上寄生,初次吸血增重为:181.30±44.35mg,两者之间有显著性差异(P<0.01)。中华硬蜱和二棘血蜱唾液腺提取物(SGE)经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),显示两者分别有24条和22条电泳带,中华硬蜱主带有6条,分子量分别为142、105、94、66/65、64和56kD,而二棘血蜱主带有5条,分子量分别为:215、114、105、66/65和58kD,经中华硬蜱叮咬致敏的家兔血清和经二棘血蜱叮咬致敏的家兔血清作免疫印渍,均显示出105kD这一电泳带。该实验表明中华硬蜱和二棘血蜱叮咬家兔两者之间存在着交叉免疫反应,提示105kD蛋白质抗原可能是两者的共同抗原。
译 名：
CROSS IMMUNE REACTION BETWEEN IXODES SINENSIS AND HAEMAPHYSALIS BISPINOSA
作 者：
LIU ZHI-GANG SONG JIE-YI PENG WEI-DONG CUI XIAO-MIN(Department of Parasitology, Jiangxi Medical College, Nanchang 330006)ZHANG YI-QIN Tu WEI-GUO Yu JIN-XING ZHOU JANG-DONG(Fengxin Sanitary and Antiepidemic Station, Fengxin County, Jiangxi Province 336300)
关键词：
Ixodes sinensis-Haema physalis bispinosa-cross immune reaction
摘 要：
A comparative study was conducted for the first time to test the cross immune reaction between rabbits sensitized by bites of Ixodes sinensis and Haemaphysalis bispinosa. When H. bispinosa fed on the rabbits which had been attacked for three times previously by /. sinensis, its body weight increased by 143.12 ± 32.67 mg. But when it fed on the controlled rabbits not attacked previously by ticks the body weight increased by 181.30 ±44.35 mg, which showed significant difference in comparison with the former case. The homogenates of /. sinensis and H. bispinosa showed 24 and 22 proteins and protein subunits respectively in the SDS-PAGE patterns. The main bands of I. sinensis were 6 and their molecular weights were 142, 105, 94, 66/65, 64 and 58 KD. The main bands of H. bispinosa were 5 and their molecular weights were 215, 114, 105, 66/65 and 52 KD. The number of protein bands of H. bispinosa and /. sinensis were recognizable by the sera of rabbits attacked by H. bispinosa and by /. sinensis, and they both contained an immunoblot, the common antigen band identified which is a 105KD protein band. It is concluded that the immune response of the rabbits attacked by these two ticks stimulates the production of antibodies which show cross immune reactions and the 105 KD protein may serve as the antigen common in both ticks.
