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当前位置: 首页 > 文章 > 松江鲈脾组织细胞的短期培养及染色体分析 大连海洋大学学报 2015,30 (2) 161-164
Position: Home > Articles > Primary culture of spleen and karyotype analysis in roughskin sculpin Trachidermus fasciatus Journal of Dalian Ocean University 2015,30 (2) 161-164

松江鲈脾组织细胞的短期培养及染色体分析

作  者：
单莉娟;向阳;李霞;王茂林;秦艳杰;吴迪;白丽雯
单  位：
大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室农业部北方海水增养殖重点实验室
关键词：
松江鲈;脾组织;体外短期培养;染色体
摘  要：
采用组织块法对体质量为120~140 g的松江鲈Trachidermus fasciatus脾组织细胞进行短期培养并对染色体进行了分析。结果表明:在p H为7.2、温度为25℃的条件下,松江鲈脾组织细胞在含有20%南美胎牛血清(FBS)、人碱性成纤维样细胞生长因子(b FGF)、硫酸软骨素、Ⅰ型胰岛素样生长因子(IGF-Ⅰ)的培养基中,生长良好,迁出细胞有悬浮和贴壁两种形式;悬浮细胞为圆形和椭圆形,贴壁细胞呈变形细胞样和成纤维细胞样;利用悬浮细胞进行的染色体分析显示,松江鲈的染色体数2n=40,核型公式为14m+10sm+16t。本研究结果为松江鲈脾组织细胞系的构建奠定了基础,并初步建立了利用短期培养的脾组织细胞制备染色体的方法。
译  名：
Primary culture of spleen and karyotype analysis in roughskin sculpin Trachidermus fasciatus
作  者：
SHAN Li-juan;XIANG Yang;LI Xia;WANG Mao-lin;QIN Yan-jie;WU Di;BAI Li-wen;Key Laboratory of Marine Bio - resource Restoration and Habitat Reparation in Liaoning Province,Key Laboratory of Mariculture & Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University;
关键词：
Trachidermus fasciatus;;spleen;;cell primary culture;;chromosome
摘  要：
Primary culture of spleen and karyotype analysis wre performed in roughskin sculpin Trachidermus fasciatus with body weight of 120- 140 g by a tissue explanting method. The results showed that the optimal growth of spleen cells in roughskin sculpin was observed in the culture medium of DMEM / F12 supplemented with 20% FBS of basic fibroblast growth factor( b FGF),insulin- like growth factor- Ⅰ( IGF- Ⅰ) and chondroitin sulfate at 25℃ and p H 7. 2. There were two types of growing spleen cells,round and oval suspended cells and cell- like and fibroblast- like adherent cells. The analysis of suspended cells revealed that roughskin sculpin had a diploid chromosome number of 40,with karyotype formula 2n = 40,and 14 m + 10 sm + 16 t. The findings provided a useful foundation for the establishment of in vitro cell- line and the functional investigation in roughskin sculpin spleen,and the chromosome preparation method was preliminarily established using cells of the spleen in roughskin sculpin.

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