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当前位置: 首页 > 文章 > 应用高效液相色谱法对饲料中五种抗生素的同时检测 现代畜牧兽医 2013 (12) 44-48
Position: Home > Articles > Simultaneous determination of many antibiotics in feedstuff by High Performance Liquid Chromatography Modern Journal of Animal Husbandry and Veterinary Medicine 2013 (12) 44-48

应用高效液相色谱法对饲料中五种抗生素的同时检测

作  者：
李丽;王金晶;陈立江;刘宇;王菁;焉喜臣
单  位：
辽宁大学药学院
关键词：
高效液相色谱法;金霉素;氯霉素;红霉素;罗红霉素;青霉素
摘  要：
本试验建立了同时测定饲料中5种抗生素含量的高效液相色谱法。试验采用梯度洗脱,使金霉素、氯霉素、红霉素、罗红霉素、青霉素得到较好的分离;柱温30℃,流速为1.0 mL/min,进样量20μL,检测波长210 nm。结果显示,金霉素、氯霉素、青霉素标准曲线线性范围为0.5~40μg/mL,红霉素、罗红霉素标准曲线线性范围为0.5~80μg/mL,相关系数r≥0.999 7。饲料中5种抗生素的加样回收率为95.86%~98.72%,RSD<2%。本试验建立的梯度洗脱方法简便快速,准确度和精密度较高,可以满足饲料中CTC、CPA、ERY、ROX、PG的检测。
译  名：
Simultaneous determination of many antibiotics in feedstuff by High Performance Liquid Chromatography
作  者：
Li Li;Wang Jinjing;Chen Lijiang;Liu Yu;Wang Jing;Yan Xichen;College of Pharmacy Liaoning University;
关键词：
HPLC;;Chlortetracycline;;Chloramphenicol;;Erythromycin;;Roxithromycin;;Benzylpenicillin potassium
摘  要：
in our study,we established an efficient method for simultaneous detection of 5 antibiotics in feed by high-performance liquid chromatography was established.Chlortetracycline(CTC),chloramphenicol(CAP),erythromycin(ERY),roxithromycin(ROX) and benzylpenicillin potassium(PG) were separated thoroughly by gradient elution under the conditions of column temperature at 30 ℃,flow rate of 1.0 mL/ min,and with the injection volume of 20 μ L.The signal was detected at 210 nm.The results showed that a linear range was found between peak height and concentration in the range of 0.5~40 μ g/mL for CTC,CAP and PG,and 0.5~80 μ g/ml.for ERY and ROX.The linear regression coefficients were not less than 0.999 7.The recovery rates of the five antibiotics were from 95.86%to 98.72%,and RSD was less than 2%. High-performance liquid chromatography is simple,rapid and has high accuracy and precision,which meets the detection of CTC,CPA,ERY,ROX and PG in feedstuff.

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