Position: Home > Articles > Purification of IgG Used for Preparing Secondary Antibody in RIA by Caprylic Acid Precipitation and DEAE Cellulose Absorption
Chinese Journal of Veterinary Science
1993
(2)
133-135
应用辛酸法-DEAE纤维素吸附过滤法提纯的IgG制备RIA用二抗
作 者：
崔青山;王志学;马海东
单 位：
兽医大学军事兽医研究所
关键词：
辛酸沉淀法;DEAE纤维素吸附过滤法;血清IgG;二抗
摘 要：
用辛酸沉淀法结合DEAE纤维素吸附过滤法,从兔、绵羊、驴和人血清中提取IgG,经PAGE和ID分析,其纯度和免疫活性较好.用提纯的兔IgG和人IgG制备的二抗,抗体效价高(驴抗兔IgG高达1024倍,羊抗人IgG达256倍),特异性好,抗体亲和力强,可供作RIA免疫分离沉淀试剂.
译 名：
Purification of IgG Used for Preparing Secondary Antibody in RIA by Caprylic Acid Precipitation and DEAE Cellulose Absorption
作 者：
Cui Qingshan et al. The Military Veterinary Institute, Veterinary College of PLA
关键词：
caprylic acid precipition;;DEAE cellulose absorption;;IgG;;secondary antibody
摘 要：
A simple two-step procedure in IgG purification from sera of human, rabbit,sheep and donkey was described. It was precipitated firstly by caprylic acid and then absorpted byDE-52 cellulose. Both the purity and immunoactivity of the resultant IgG were proved to bebetter by PAGE and ID. It was used to immunize the heterologous animals and secondary anti-bodies with high titer, good specificity and potent affinity were obtained.
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