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Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

作者：

Yuyu Chen;Anning Zhu;Peng Xue;Xiaopeng Wen;Yang Cao;Beifang Wang;Yue Zhang;Liaqat Ali Shah;Shifeng Cheng;Yingxin Zhang;Yingxin Zhan

单位：

State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China; State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China;National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, Chin;Nanchong Academy of Agricultural Sciences, Nanchong 637000, China

关键词：

rice;GS3;GL3.1;grain size;grain quality;grain yiel

摘要：

Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T-1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3g13.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3g13.1 mutants were affected. It is noteworthy that gs3 and gs3g13.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.

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Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

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