中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria 农业科学学报 (英文) 2021,20 (10)

Position: Home > Articles > Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria Journal of Integrative Agriculture 2021,20 (10)

Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria

作者：

Daramola, Fisayo Y.;Osemwegie, Osarenkhoe O.;Owa, Stephen O.;Orisajo, Samuel B.;Ikponmwosa, Evbuomwan;Alori, Elizabeth T.

单位：

Landmark Univ, Dept Crop & Soil Sci, Omu Aran 370102, Kwara State, Nigeria;Cocoa Res Inst Nigeria, Crop Protect Div, Ibadan 200257, Oyo State, Nigeria;Landmark Univ, Dept Microbiol, Omu Aran 370102, Kwara State, Nigeria

关键词：

biological control;EPNs;Heterorhabditis sp;ribosomal DNA;Tenebrio molitor

摘要：

The occurrence of entomopathogenic nematodes (EPNs) in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait (White's trap) techniques. Isolates were tested for infectivity using the larvae of Galleria mellonella (greater moth) and Tenebrio molitor (mealworm). The study revealed a new species of Heterorhabditis (MT371593) in soil samples that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University, Nigeria. Amplification of the internal transcribed spacer region (ITS) of the ribosomal DNA produced a nucleotide sequence of 933 base pairs (bp). A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99% similarity to that of Heterorhabditis sp. from Thailand. Infectivity test of the isolate showed 100% mortality against T. molitor larvae within 48 h of exposure while only 80% mortality was recorded for G. mellonella after 1 week of exposure. This is the first account of Heterorhabditis sp. in Nigeria. The varying degrees of infectivity against mealworm and greater moth observed in this study proved that the Nigerian isolate of Heterorhabditis sp. could potentially be an attractive option in the management of insect pests of cash crops.

相似文章

Bacillus cereus X5 enhanced bio-organic fertilizers effectively control root-knot nematodes (Meloidogyne sp.). [Xiao TongJian, Chen Fang, Gao Chao, Zhao QingYun, Shen QiRong, Ran Wei] 土壤圈(英文版) 2013,23 (2)
Screening of Antagonistic Bacteria from Phyllosphere towards Tobacco Brown Spot Fungus Alternaria alternata [ZHANG Chengsheng, KONG Fanyu, LI Duochuan, WANG Jing, WANG Fenglong] 东北农业大学学报(英文版) 2006,13 (1)
Arbuscular mycorrhizal fungal inoculation enhances suppression of cucumber Fusarium wilt in greenhouse soils. [Hu JunLi, Lin XianGui, Wang JunHua, Shen WeiShou, Wu Shu, Peng SuPing, Mao TingTing] 土壤圈(英文版) 2010,20 (5)
Repellent Activity of Extracts of Wild Rice Species against Panonychus citri and Aphis citricola in Associated with Esterase Isoenzyme in Insests [WAN Shu-qing, LIU Xiang-fa, FENG Guo-zhong, PAN Da-jian] 水稻科学(英文版) 2006,13 (2) 146-148
Control efficacy and antifungal mechanism of Bacillus cereus strain JK14 against wheat take-all disease. [Han YanXia, Hu BinJie, Wang GongNan] 农业科学与技术(英文版) 2008,9 (1) 70-74
Evaluation of Curvularia lunata Strain B6 as a Potential Mycoherbicide to Control Barnydrgrass (Echinochloa crus-galli) [Li Jing, Wei Tao, Sun Ai-rui, Ni Han-wen] 农业科学学报 (英文) 2013,12 (7)
Herbicidal activity of Aureobasidium pullulans PA-2 on weeds and optimization of its solid-state fermentation conditions [Guo Qing-yun, Cheng Liang, Zhu Hai-xia, Li Wei, Wei You-hai, Chen Hong-yu, Guo Liang-zhi, Weng Hua, Wang Jian] 农业科学学报 (英文) 2020,19 (1)
Overwintering parasitism is positively associated with population density in diapausing larvae of Chilo suppressalis [Huang Xiao-long, Jiang Ting, Wu Zhen-ping, Zhang Wan-na, Xiao Hai-jun] 农业科学学报 (英文) 2020,19 (3)
High temperatures do not decrease biocontrol potential for the host-killing parasitoid Neochrysocharis formosa (Hymenoptera: Eulophidae) on agromyzid leafminers [XUAN, Jing-li, XIAO, Yue, YE, Fu-yu, ZHANG, Yi-bo, TAO, Shu-xia, GUO, Jian-yang, LIU, Wan-xue] 农业科学学报 (英文) 2022,21 (6)
Susceptibility and tissue specificity of Spodoptera frugiperda to Junonia coenia densovirus [Chen Zu-wen, Yang Yan-chao, Zhang Jian-feng, Jin Ming-hui, Xiao Yu-tao, Xia Zhi-chao, Liu Yuan-yuan, Yu Sai-zhen, Yang Yong-bo, Wang Yuan, Li Yi, Liu Kai-yu] 农业科学学报 (英文) 2021,20 (3)

PDF下载 PDF预览

导出引用

计量

文章访问数: 6

HTML全文浏览量: 0

PDF下载量: 0

Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)