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当前位置: 首页 > 文章 > Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa 作物学报(英文版) 2021,9 (5)
Position: Home > Articles > Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa The Crop Journal 2021,9 (5)

Heterogeneous expression of Osa-MIR156bc increases abiotic stress resistance and forage quality of alfalfa

作  者：
Kexin Wang;Yanrong Liu;Fengkui Teng;Huifang Cen;Jianpin Yan;Shiwen Lin;Dayong Li;Wanjun Zhan
单  位：
College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, China;College of Biological Science, China Agricultural University, Beijing 100193, China;Key Lab of Grassland Science in Beijing, China Agricultural University, Beijing 100193, Chin;College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
关键词：
Alfalfa;miR156;Biomass yield;Forage quality;Abiotic stres
摘  要：
Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop woldwide. MicroRNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant architecture and abiotic stress resistance. However, there has been no report on whether a miR156 precursor gene from a monocotyledonous species functions in alfalfa. We introduced two tandem precursor genes of miR156, rice Osa-MIR156b and Osa-MIR156c (Osa-MIR156bc), into alfalfa. The expression of miR156 in the transgenic (TG) alfalfa was significantly elevated. Compared to wild-type plants, the TG plants overexpressing miR156 had more branches and leaves and showed improved salt and drought tolerance. Overexpression of miR156 slightly reduced plant height, but the biomass yield of TG plants grown in flowerpots was still increased. Forage quality of TG plants was markedly improved by reduction of acid detergent lignin (ADL) content and increase in crude protein content. The expression of the putative miR156 target genes MsSPL6, MsSPL12, and MsSPL13 in TG plants was repressed by miR156 overexpression, and that of all tested MsSPLs would be sharply increased under drought or salt stress. RNA sequencing revealed that overexpression of miR156 affected the expression of genes associated with abiotic stress resistance and plant development in multiple pathways. This first report of overexpression of monocot miR156 precursors in alfalfa sheds light on the function of miRNA156 precursors from the monocot species rice that could be used for genetic improvement of the dicot forage crop alfalfa. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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