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当前位置: 首页 > 文章 > Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing 作物学报(英文版) 2021,9 (6)
Position: Home > Articles > Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing The Crop Journal 2021,9 (6)

Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing

作  者：
Yang Liu;Hui Chen;Chunxin Li;Lirong Zhang;Mingqin Shao;Yuhui Pang;Xiangyang Xu;Guihua Ba
单  位：
Dept. of Agronomy Kansas State University Manhattan KS 66506 USA;College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei, China; Institute of South Subtropical Crops, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, Guangdong, China;USDA‐ARS, Wheat, Peanut, and Other Field Crop Research Unit Stillwater OK 74075 USA; Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA;Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA;College of Agriculture, Henan University of Science and Technology, Luoyang 471023, Henan, China; USDA-ARS, Hard Winter Wheat Genetic Research Unit, Manhattan, KS 66506, US
关键词：
Aegilops tauschii;Triticum tauschii;Lr42;KASP;Diagnostic marke
摘  要：
Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene (TaRPM1) encoding a putative NBARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. Markers were designed based on a deletion mutation and a single nucleotide polymorphism (SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high- or low-throughput marker screening facilities. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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