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Position: Home > Articles > Analysis of DNA methylation of CD79B in MDV-infected chicken spleen Journal of Integrative Agriculture 2021,20 (11)

Analysis of DNA methylation of CD79B in MDV-infected chicken spleen

作  者:
Wang Lu-Lu;Zhao Chun-Fang;Liu Chang-Jun;Zhang Hao;Lian Ling
单  位:
China Agr Univ, Natl Engn Lab Anim Breeding, Coll Anim Sci & Technol, Beijing 100193, Peoples R China;China Agr Univ, MOA Key Lab Anim Genet & Breeding, Coll Anim Sci & Technol, Beijing 100193, Peoples R China;Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Avian Infect Dis, Harbin 150001, Peoples R China
关键词:
chicken;Marek's disease;Marek's disease virus;DNA methylation;gene expression
摘  要:
Marek's disease (MD), an immunosuppressive disease induced by Marek's disease virus (MDV), provides an ideal model for studying diseases caused by a carcinogenic virus. CD79B is a B-cell antigen receptor complex-associated protein beta-chain precursor which is involved in the activation, proliferation, differentiation of B-cell and the transmission of downstream signals. This study analyzed CD79B gene mRNA expression and methylation by two schemes #20 (5 ' flanking to intron 1) and #27 (intron 2 to intron 3), between MDV-infected tumorous spleens (TS) and non-infected spleens (NS). Results showed that average methylation levels of CpGs in #20 and #27 were higher in TS than in NS (P<0.05), while, CD79B mRNA expression was lower in TS than in NS (P<0.01). Six of 40 CpG sites showed significantly (P<0.05) different methylation levels between TS and NS. Correlation analysis showed that the average methylation level rather than a single site methylation level in #20 affected (P<0.05) mRNA expression. Collectively, it was found that the change of CD79B gene expression after MDV infection might be partly explained by modification of DNA methylation.

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