Shengnan Ge;Debin Liu;Min Chen;Xinyu Liu;Yulei Wei;Xinyang Che;Lei Zhu;Lin He;Jingyu X

Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province/Key Laboratory of Low Carbon Green Agriculture in Northeast Plain, Ministry of Agriculture and Rural Affairs, China/Research Center of Saline and Alkali Land Improvement Engineering Technology in Heilongjiang Province/College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China; National Coarse Cereal Engineering Research Center, Daqing 163319, Heilongjiang, Chin

Sorghum ( Sorghum bicolor L;Moench);Membrane lipid remodeling;Lipidome;Transcriptome;Salt stress;GENETIC-CHARACTERIZATION;MUTANTS DEFICIENT;METABOLISM;CHLOROPLAST;TOLERANCE;PHOSPHATIDYLCHOLINE;DEGRADATION;MECHANISMS;SALINITY;PATHWA

Salt stress limits plant growth and development. In this study, changes in membrane lipids were inves-tigated in leaves of sorghum seedlings subjected to salt stress (150 mmol L-1 NaCl). Galactolipids (DGDG and MGDG) accounted for more than 65% of the total glycerolipids in sorghum leaves. The predominance of C36 molecular species in MGDG suggested that sorghum is an 18:3 plant. Under NaCl treatment, the content of major phospholipids (PC and PE) increased, accompanied by the activation of their metabolism pathways at the transcriptional level. In contrast, the proportion of MGDG and PG dropped drastically, leading to a decreased ratio of plastidic to non-plastidic lipids. An adjustment of glycerolipid pathway between the cytosolic and plastidic compartments was triggered by salt stress, as reflected by the increased conversion of PC to PA, providing precursors for galactolipid synthesis. The elevated DGDG resulted in increased DGDG/MGDG and bilayer/non-bilayer lipid ratios. The double-bond index of PC, PE, and DGDG increased markedly, evidently owing to the increased expression of FAD3 and FAD8. These findings will be helpful for understanding dynamic membrane lipid changes and adaptive lipid remodeling in sorghum response to salt stress.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).