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Position: Home > Articles > Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD Journal of Integrative Agriculture 2022,21 (10)

Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

作者：

Li, Chen;Liu, Xuan-xuan;Abouelnasr, Hesham;Mohamed Hamed, Arisha;Kou, Meng;Tang, Wei;Yan, Hui;Wang, Xin;Wang, Xiao-xiao;Zhang, Yun-gang;Liu, Ya-ju;Gao, Run-fei;Ma, Meng;Li, Qiang

单位：

Zagazig Univ, Fac Agr, Dept Hort, Zagazig 44511, Egypt;Chinese Acad Agr Sci, Xuzhou Inst Agr Sci Jiangsu Xuhuai Dist, Sweetpotato Res Inst, Key Lab Biol & Genet Breeding Sweetpotato,Minist A, Xuzhou 221131, Peoples R China;Natl Res Ctr, Plant Pathol Dept, Agr & Biol Res Div, Giza 12622, Egypt;Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China

关键词：

sweetpotato;miR397;STTM;SPVD;lignin content

摘要：

As a critical food crop, sweetpotato (Ipomoea batatas (L.) Lam.) is widely planted all over the world, but it is deeply affected by Sweetpotato Virus Disease (SPVD). The present study utilized short tandem target mimic (STTM) technology to effectively up-regulate the expression of laccase (IbLACs) by successfully inhibiting the expression of miR397. The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation, including phenylalanine ammonialyase (PAL), 4-coumarate-CoAligase (4CL), hydroxycinnamoyl CoA:shikimatetransferase (HTC), caffeicacid O-methyltransferase (COMT), and cinnamyl alcohol dehydrogenase (CAD). Meanwhile, the activities of PAL and LAC increased significantly, finally leading to increased lignin content. Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants, reduced the accumulation of SPVD transmitted by Bemisia tabaci (Gennadius), and promoted healthy sweetpotato growth. The results provide new insights for disease resistance breeding and green production of sweetpotato.

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Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

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