中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize 作物学报(英文版) 2023,11 (1)
Position: Home > Articles > Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize The Crop Journal 2023,11 (1)

Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize

作  者：
Pingxi Wang;Yuan Yang;Dongdong Li;Jialiang Xu;Rong Gu;Jun Zheng;Junjie Fu;Jianhua Wang;Hongwei Zhan
单  位：
National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan, Chin; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan, China; College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Center for Seed Science and Technology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词：
Maize;Plant height;ZmAMP1;Green revolutio
摘  要：
Gene resources associated with plant stature and flowering time are invaluable for maize breeding. In this study, using an F2:3 population derived from a natural semi-dwarf mutant grmm and a normal inbred line Si 273, we identified a major pleiotropic QTL on the distal long arm of chromosome 1 (qPH1_dla), and found that qPH1_dla controlled plant height, flowering time, ear and yield traits. qPH1_dla was fine -mapped to a 16 kb interval containing ZmAMP1, which was annotated as a glutamate carboxypeptidase. Allelism tests using two independent allelic mutants confirmed that ZmAMP1 was the causal gene. Real-time quantitative PCR and genomic sequence analysis suggested that a nonsynonymous mutation at the 598th base of ZmAMP1 gene was the causal sequence variant for the dwarfism of grmm. This novel ZmAMP1 allele was named ZmAMP1_grmm. RNA sequencing using two pairs of near isogenic lines (NILs) showed that 84 up-regulated and 68 down-regulated genes in dwarf NILs were enriched in 15 metabolic pathways. Finally, introgression of ZmAMP1_grmm into Zhengdan 958 and Xianyu 335 gener-ated two improved F1 lines. In field tests, they were semi-dwarf, early-flowering, lodging-resistant, and high-yielding under high-density planting conditions, suggesting that ZmAMP1_grmm is a promising Green Revolution gene for maize hybrid breeding.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

相似文章

PDF下载 PDF预览
导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46