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Position: Home > Articles > See the color, see the seed: GmW1 as a visual reporter for transgene and genome editing in soybean The Crop Journal 2023,11 (1)

See the color, see the seed: GmW1 as a visual reporter for transgene and genome editing in soybean

作者：

Li Chen;Shan Yuan;Yupeng Cai;Weiwei Yao;Qiang Su;Yingying Chen;Jialing Zhang;Wensheng Ho

单位：

Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;National Center for Transgenic Research in Plants, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;National Center for Transgenic Research in Plants, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, Chin

关键词：

Soybean;GmW1;Color;Transgenic lines;Genome editin

摘要：

A fast and efficient recognition method of transgenic lines will greatly improve detection efficiency and reduce cost. In this study, we successfully identified the transgenic soybean plants by the color. We iso-lated a GmW1 gene encoding a flavonoid 3'5'-hydroxylase from a soybean cultivar ZH42 (purple flower). We found that purple flowers occurred in the overexpression lines in the Jack and Williams 82 back-grounds (white flower). All plants with purple flowers were positive, and this trait seems stably inherited in the offspring. We have also obtained the editing plants, which were classified into three types accord-ing to the different flower colors appeared. We analyzed the phenotype and the homozygous types of the T1 mutants. We also found that a correspondence between flower color and stem color. This study pro-vides a visible color reporter on soybean transformation. It can be quickly and early to identify the trans -genic soybean plants by stem color of seedlings, which substantially reduces the amount of labor and cost.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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See the color, see the seed: GmW1 as a visual reporter for transgene and genome editing in soybean

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