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Position: Home > Articles > Functional analysis of MdSUT2.1, a plasma membrane sucrose transporter from apple Journal of Integrative Agriculture 2023,22 (3)

Functional analysis of MdSUT2.1, a plasma membrane sucrose transporter from apple

作者：

Bo, Zhang;Wen-min, Fan;Zhen-zhen, Zhu;Ying, Wang;Zheng-yang, Zhao

单位：

Shaanxi Res Ctr Apple Engn & Technol, Yangling 712100, Peoples R China;Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China

关键词：

apple;MdSUT2;1;sugar;transport;plasma membrane

摘要：

Sugar content is a determinant of apple (Malusxdomestica Borkh.) sweetness. However, the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive. Herein, this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in apples. The MdSUT2.1 gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf protoplasts. MdSUT2.1 was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit development. Moreover, complementary growth assays in a yeast mutant validated the sucrose transport activity of MdSUT2.1. MdSUT2.1 overexpression in apples and tomatoes resulted in significant increases in sucrose, fructose, and glucose contents compared to the wild type (WT). Further analysis revealed that the expression levels of sugar metabolism-and transport-related genes SUSYs, NINVs, FRKs, HXKs, and TSTs increased in apples and tomatoes with MdSUT2.1 overexpression compared to WT. Finally, unlike the tonoplast sugar transporters MdTST1 and MdTST2, the promoter of MdSUT2.1 was not induced by exogenous sugars. These findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples.

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Functional analysis of MdSUT2.1, a plasma membrane sucrose transporter from apple

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