中国农业期刊集成服务平台

当前位置: 首页 > 文章 > BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus 作物学报(英文版) 2023,11 (2)

Position: Home > Articles > BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus The Crop Journal 2023,11 (2)

BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

作者：

Ran Hu;Meichen Zhu;Si Chen;Chengxiang Li;Qianwei Zhang;Lei Gao;Xueqin Liu;Shuai Shen;Fuyou Fu;Xinhua Xu;Ying Liang;Liezhao Liu;Kun Lü;Hao Yu;Jiana Li;Cunmin Q

单位：

Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China;Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 02X, Canada; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, Chin;Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore;Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117558, Singapore; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China

关键词：

Brassica napus L.;BnbHLH92a;Anthocyanin;Proanthocyanidins;Flavonoid pathwa

摘要：

Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed, and anthocyanin and proanthocyanidins (PAs) are major seed-coat pigments. Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed. In this study, we identified a transcription factor gene BnbHLH92a (BnaA06T0441000ZS) in rapeseed. Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs. Correspondingly, the expression profiles of anthocyanin and PA biosynthesis genes (TT3, BAN, TT8, TT18, and TTG1) were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds, indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis. BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed. BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression. These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B. napus. (C) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

相似文章

Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A [Dong Yun, Wang Yi, Jin Feng-wei, Xing Li-juan, Fang Yen, Zhang Zheng-ying, Zou Jun-jie, Wang Lei, Xu Miao-yun] 农业科学学报 (英文) 2020,19 (7)
cDNA-Amplified fragment length polymorphism analysis reveals differential gene expression induced by exogenous MeJA and GA₃ in oilseed rape (Brassica napus L.) flowers [Pak, Haksong, Li Yu-ling, Kim, Hyenchol, Jiang Li-xi] 农业科学学报 (英文) 2017,16 (1)
5-Aminolevulinic acid alleviates herbicide-induced physiological and ultrastructural changes in Brassica napus [Xu Ling, Islam, Faisal, Zhang Wen-fang, Ghani, Muhammad A., Ali, Basharat] 农业科学学报 (英文) 2018,17 (3)
Detection of new candidate genes controlling seed weight by integrating gene coexpression analysis and QTL mapping in Brassica napus L. [Hongli Dong, Lei Yang, Yilin Liu, Guifu Tian, Huan Tang, Shuangshuang Xin, Yixin Cui, Qing Xiong, Heng Wan, Zhi Li, Christian Jung, Wei Qia] 作物学报(英文版) 2023,11 (3)
Dissecting the genetic architecture of glucosinolate compounds for quality improvement in flowering stalk tissues of Brassica napus [Changbin Gao, Fugui Zhang, Yang Hu, Liping Song, L. Tang, Xueli Zhang, Cong’an He, Aihua Wang, Xiaoming W] 园艺学报(英文版) 2023,9 (3)
BnaSD.C3 is a novel major quantitative trait locus affecting semi- dwarf architecture in Brassica napus L [Wang, Xiao-dong, Cai, Ying, Pang, Cheng-ke, Zhao, Xiao-zhen, Shi, Rui, Liu, Hong-fang, Chen, Feng, Zhang, Wei, Fu, San-xiong, Hu, Mao-long, Hua, Wei, Zheng, Ming, Zhang, Jie-fu] 农业科学学报 (英文) 2023,22 (10)
Physiological and molecular responses to cold stress in rapeseed (Brassica napus L.) [Yan Lei, Shah, Tariq, Cheng Yong, Lu Yan, Zhang Xue-kun, Zou Xi-ling] 农业科学学报 (英文) 2019,18 (12)
Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector [Hussain, Nazim, Jabeen, Zahra, Li Yuan-long, Chen Ming-xun, Li Zhi-lan, Guo Wan-li, Shamsi, Imran Haider, Chen Xiao-yang, Jiang Li-xi] 农业科学学报 (英文) 2013,12 (5)
Molecular evidence for blocking erucic acid synthesis in rapeseed (Brassica napus L.) by a two-base-pair deletion in FAE1 (fatty acid elongase 1) [Wu Lei, Jia Yan-li, Wu Gang, Lu Chang-ming] 农业科学学报 (英文) 2015,14 (7)
Physiological and molecular responses to cold stress in rapeseed (Brassica napus L.) [Lei Yan, Tariq Shah, Yongqiang Cheng, Lu Yan, Xuekun Zhang, Xiling Zo] 农业科学学报(英文版) 2019,18 (12)

PDF下载 PDF预览

导出引用

计量

文章访问数: 5

HTML全文浏览量: 0

PDF下载量: 0

BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

相似文章

所属期刊

作物学报(英文版)

推荐期刊

农业科学学报 (英文)