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Position: Home > Articles > A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley (Hordeum vulgare L.) Journal of Integrative Agriculture 2023,22 (8)

A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley (Hordeum vulgare L.)

作者：

Guo, Bao-jian;Sun, Hong-wei;Qi, Jiang;Huang, Xin-yu;Hong, Yi;Hou, Jian;Lu, Chao;Wang, Yu-lin;Wang, Fei-fei;Zhu, Juan;Guo, Gang-gang;Xu, Ru-gen

单位：

Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Gene Resources & Genet Improvement, Key Lab Crop Germplasm Utilizat,Minist Agr, Beijing 100081, Peoples R China;Tibet Acad Agr & Anim Husb Sci TAAAS, State Key Lab Barley & Yak Germplasm Resources &, Lhasa 850002, Peoples R China;Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Jiangsu Key Lab Crop Genom & Mol Breeding, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China

关键词：

barley;EMS;plastochron;many noded dwarf;MATE transporter;shoot architecture

摘要：

In higher plants, the shoot apical meristem produces lateral organs in a regular spacing (phyllotaxy) and timing (plastochron). The molecular analysis of mutants associated with phyllotaxy and plastochron would increase our understanding of the mechanism of shoot architecture formation. In this study, we identified mutant mnd8ynp5 that shows an increased rate of leaf emergence and a larger number of nodes in combination with a dwarfed growth habit from an EMS-treated population of the elite barley cultivar Yangnongpi 5. Using a map-based cloning strategy, the mnd8 gene was narrowed down to a 6.7-kb genomic interval on the long arm of chromosome 5H. Sequence analysis revealed that a C to T single-nucleotide mutation occurred at the first exon (position 953) of HORVU5Hr1G118820, leading to an alanine (Ala) to valine (Val) substitution at the 318th amino acid site. Next, HORVU5Hr1G118820 was defined as the candidate gene of MND8 encoding 514 amino acids and containing two multidrug and toxic compound extrusion (MATE) domains. It is highly homologous to maize Bige1 and has a conserved function in the regulation of plant development by controlling the leaf initiation rate. Examination of modern barely varieties showed that Hap-1 was the dominant haplotype and was selected in barley breeding around the world. Collectively, our results indicated that mnd8ynp5 is a novel allele of the HORVU5Hr1G118820 gene that is possibly responsible for the shortened plastochron and many noded dwarf phenotype in barley.

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A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley (Hordeum vulgare L.)

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