JIANG, Yun;WANG, De-li;HAO, Ming;ZHANG, Jie;LIU, Deng-cai

Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu 610061, Peoples R China;Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Peoples R China;Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu 611130, Peoples R China

Aegilops kotschyi;common wheat;1U(k)(1A) substitution line;HMW-GS;processing quality

Exploring novel high molecular weight glutenin subunits (HMW-GSs) from wild related species is a strategy to improve wheat processing quality. The objective of the present investigation was to identify the chromosomes of the wheat -alien introgression line N124, derived from the hybridization between Triticum aestivum with Aegilops kotschyi, and characterize the effects on quality-related traits. Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1U(k)(1A) substitution line. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed -phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae. kotschyi parent. PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were U(k)x and U(k)y. Compared to the wheat parent, the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters. It can be served as a donor or bridge material for wheat quality improvement.