Yutian Gao;Xiaohong Tian;Weidong Wang;Xiangru Xu;Yubin Su;Jiatian Yang;Shuonan Duan;Jinlong Li;Mingming Xin;Huiru Peng;Qixin Sun;Chaojie Xie;Jun M

Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, Hebei, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, Chin;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

Fusarium crown rot (FCR);Wheat;Hormone;Root;TaOPR

Fusarium crown rot (FCR) is a soilborne disease causing severe yield losses in many wheat-growing areas of the world. Diseased plants show browning and necrosis of roots and stems causing white heads at maturity. Little is known about the molecular processes employed by wheat roots to respond to the dis-ease. We characterized morphological, transcriptional and hormonal changes in wheat seedling roots fol-lowing challenge with Fusarium pseudograminearum (Fp), the main pathogen of FCR. The pathogen inhibited root development to various extents depending on plants' resistance level. Many genes respon-sive to FCR infection in wheat roots were enriched in plant hormone pathways. The contents of com-pounds involved in biosynthesis and metabolism of jasmonic acid, salicylic acid, cytokinin and auxin were drastically changed in roots at five days post-inoculation. Presoaking seeds in methyl jasmonate for 24 h promoted FCR resistance, whereas presoaking with cytokinin 6-benzylaminopurine made plants more susceptible. Overexpression of TaOPR3, a gene involved in jasmonic acid biosynthesis, enhanced plant resistance as well as root and shoot growth during infection.(c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).