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当前位置: 首页 > 文章 > Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat 作物学报(英文版) 2023,11 (5)
Position: Home > Articles > Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat The Crop Journal 2023,11 (5)

Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

作  者：
Caiyun Liu;Wangzhen Guo;Yan Wang;Bisheng Fu;Jaroslav Doležel;Ying Liu;Wenling Zhai;Mahmoud Said;István Molnár;Kateřina Holušová;Ruiqi Zhang;Jizhong W
单  位：
Institute of Experimental Botany, Centre of the Haná Region for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-783671, Olomouc, Czech Republic; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Zhongshan Biological Breeding Laboratory, Nanjing 210014, Jiangsu, Chin; Field Crops Research Institute, Agricultural Research Centre, 9 Gamma Street, Giza, 12619 Cairo, Egypt; Institute of Experimental Botany, Centre of the Haná Region for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-783671, Olomouc, Czech Republic;Institute of Germplasm Resources and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China;Agricultural Institute, Centre for Agricultural Research, ELKH, Martonvásár 2462, Hungary;College of Agronomy/JCIC-MCP/National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China;Field Crops Research Institute, Agricultural Research Centre, 9 Gamma Street, Giza, 12619 Cairo, Egypt
关键词：
Dasypyrum villosum;Flow sorting;KASP;Rhizoctonia cerealis;Triticum aestivu
摘  要：
Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven, has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat-Dasypyrum villosum T2DS.2V#4L translocation line in the background of Chinese Spring (CS) showed stable resistance to R. cerealis. Introgression of the T2DS.2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS.2V#4L in comparison with NIL-T2DS.2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3, and BC2F2:4 generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and 8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and 2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and 30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat back-grounds. T2DS.2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.(c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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