Ye Wangbin;Wang Ling;Zhou Sheng;Yang Juan;Liu Guiling;Fan Lijuan;Northeast Forestry University;

Iris laevigata;;IlWRKY11 gene;;Subcellular localization

In order to develop functional gene resources of WRKY family of Iris laevigata, verify the relevance of WRKY11 transcription factor to flowering regulation and better serve I. laevigata targeted molecular breeding. The I. laevigata from the Northeast Forestry University was used as the material, and the I. laevigata tepals were used as cDNA templates to clone the IlWRKY11 gene, perform bioinformatics analysis and subcellular localization of IlWRKY11 protein structure, and the functional elucidation of WRKY11 transcription factor on flowering regulation by transforming the model plant Arabidopsis thaliana was conducted with this gene. The results showed that the cDNA reverse-transcribed from total RNA of I. laevigata was used as a template to clone the IlWRKY11 gene with a length of 888 bp and encoding 295 amino acids. The relative molecular mass of IlWRKY11 protein is 32 291 kDa and the molecular formula is C_(1398)H_(2237)N_(419)O_(421)S_(20), belonging to unstable hydrophilic protein. The pCAMBIA1300-IlWRKY11-GFP plant overexpression vector determined that IlWRKY11 protein is localized in the nucleus, and IlWRKY11 protein is more closely related to the monocotyledonous plants such as Apostasia shenz-henica and Asparagus officinalis. Bolting time and flowering time are 2-3 d earlier in IlWRKY11 gene overexpressed arabidopsis compared in the control. The IlWRKY11 gene is found to be involved in flowering regulation through regulation of the gibberellin pathway-related gene GA20OX and the vernalization pathway-related gene VRN1 as well as the age pathway-related gene SPL3 by qRT-PCR assay of flowering-phase related gene expression in transgenic arabidopsis and trans-null control. It is suggested that the IlWRKY11 gene(ON399551) plays an important role in regulating blooming time for I. laevigata.