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Position: Home > Articles > Comparison of Morphological and SRAP Markers in Watermelon (Citrullus lanatus) DUS Test Journal of Plant Genetic Resources 2011 (5) 790-795

SRAP标记与形态学标记在西瓜DUS测试中的比较

作  者:
刘丽娟;舒烈波;罗利军;陈海荣
单  位:
上海市农业生物基因中心
关键词:
DUS测试;遗传多样性;SRAP标记;形态学标记
摘  要:
以28个西瓜品种为研究对象,分别采用21对SRAP引物标记和54个用于DUS测试的形态学标记对其进行遗传多样性分析,其中SRAP引物在不同品种间的多态信息含量(PIC)在52.5%~89.2%之间,平均值为72.0%,计算得到的各材料间相似系数在0.92至0.99之间,而形态学标记统计得到各材料间相关系数在0.50到0.85之间。采用UPGMA法对所有材料进行聚类分析,SRAP分子标记聚类划分成4类,形态学标记将其划分为5类。对两种标记所得的结果进行相关性分析得出两者的相关系数为0.218,表明形态学标记和SRAP标记在这些材料上表现的相关性不是很高,但在品种鉴定和区别上SRAP标记表现出一定的优势,可以作为DUS测试的一种有益补充。
译  名:
Comparison of Morphological and SRAP Markers in Watermelon (Citrullus lanatus) DUS Test
作  者:
LIU Li-juan1,2,SHU Lie-bo1,2,LUO Li-jun1,2,CHEN Hai-rong1(1 Shanghai Agrobiological Gene Center,Shanghai 201106;2 National Key Laboratory of Crop Genetic Improvement/College of Plant Science.Huazhong Agricultural University,Wuhan 430070)
关键词:
DUS test;Genetic diversity;SRAP;Morphological traits
摘  要:
The tests of distinctiveness,uniformity,and stability(DUS) are necessary steps for indentification and authorizatian of new varieties.Twenty-one SRAP markers and 54 morphological traits for DUS tests were used for diversity analysis of 28 watermelon(Citrullus lanatus) varieties.Average polymorphism information content(PIC) of the SRAP markers was 0.72,ranging from 52.5% to 89.2% and the correlation coefficients of varieties ranged from 0.92 to 0.99.The correlation coefficients ranged from 0.50 to 0.85 in the morphological traits test.Cluster analysis of the banding patterns using UPGMA analysis separated the varieties into four(SRAP markers) and five(morphological) groups.The correlation coefficient between two markers was 0.218.showing no significance correlation.But the SRAP markers showed some advantages in varieties identification,which indicates to be appropriate supplement for DUS test.

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