Position: Home > Articles > Determination of Daminozide Residue in Fruits by Liquid Chromatography-ESI Tandem Mass Spectrometry
Agrochemicals
2010,49
(3)
191-193
液相色谱-电喷雾串联四极杆质谱测定水果中的丁酰肼
作 者：
余苹中;宋稳成;李雪生;潘灿平;刘丰茂
单 位：
农业部农药检定所;中国农业大学理学院;北京市农林科学院植保环保研究所
关键词：
液相色谱-电喷雾串联四极杆质谱法;水果;丁酰肼;残留
摘 要：
建立了4种水果中丁酰肼残留量的液相色谱-电喷雾串联四极杆质谱检测方法。样品经匀浆提取净化后,采用LC-MS/MS法,ESI(+)电离方式,多反应监测(MRM)定量,用Vensil HILIC色谱柱,以含0.1%甲酸的水和乙腈为流动相进行洗脱。结果表明:丁酰肼在0.01～1.0mg/L质量浓度范围内呈现良好的线性,相关系数为0.9991,方法的检出限为2.5μg/kg,当添加质量分数为0.01、0.1、0.5mg/kg时,4种水果中丁酰肼的平均回收率为77.7%～103.7%,相对标准偏差为2.0%～9.3%。
译 名：
Determination of Daminozide Residue in Fruits by Liquid Chromatography-ESI Tandem Mass Spectrometry
作 者：
YU Ping-zhong1,2,3,SONG Wen-cheng4,LI Xue-sheng2,PAN Can-ping2,LIU Feng-mao2(1.Institute of Plant Protection and Environmental Protection,Academy of Beijing Agricultural Science,Beijing 100097,China;2.College of Science,China Agricultural University,Beijing 100193,China;3.Beijing Research Center for Agrifood Testing and Farmland Monitoring,Beijing 100097,China;4.Institute for Control of Agrochemicals,Ministry of Agriculture,Beijing 100125,China)
关键词：
LC-MS/MS;fruit;daminozide;residue
摘 要：
A method for determination of daminozide residue in four fruits by liquid chromatography-ESI tandem mass spectrometry(LC-MS/MS) has been developed.After sample was extracted and cleaned up,daminozide was detemined by LC/MS/MS-ESI(+),quantified with multiple reaction monitoring(MRM) mode.The separation was performed on a Vensil HILIC column with a gradient system of water(containing 0.1% formic acid)-acetonitril as mobile phase.The results showed that the calibration linear range of daminozide was from 0.01 to 1.0 mg/L with a correlation coefficient of 0.9991.The detection limit was 2.5 μg/kg.Average recoveries(spiked at the levels of 0.01,0.1,0.5 mg/kg) range from 77.7-103.7%,the relative standard deviations was between 2.0 and 9.3%.
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