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当前位置: 首页 > 文章 > 鸡血红素加氧酶-1(HO-1)和胆绿素还原酶A(BLVRA)基因表达量对蛋壳颜色的影响 中国农学通报 2014,30 (35) 64-68
Position: Home > Articles > The Effects of the Expression Levels of H0-1 and BLVRA on the Color of Egg Shells Chinese Agricultural Science Bulletin 2014,30 (35) 64-68

鸡血红素加氧酶-1(HO-1)和胆绿素还原酶A(BLVRA)基因表达量对蛋壳颜色的影响

作  者：
王晓庆;徐廷生;雷雪芹;高灵照;肖赞奇
单  位：
河南科技大学动物科技学院;卢氏县畜牧局
关键词：
鸡;血红素加氧酶;胆绿素还原酶A;实时荧光定量PCR;蛋壳颜色
摘  要：
研究旨在检测血红素加氧酶(HO-1)和胆绿素还原酶A(BLVRA)在卢氏鸡和固始鸡输卵管子宫部的表达量差异,探索绿壳蛋的形成机理。以24周龄产绿壳蛋卢氏鸡和产褐壳蛋固始鸡为素材,采集每只鸡输卵管子宫部,提取总RNA,经过RT-PCR反转录为c DNA,以与色素相关的血红素加氧酶-1(Heme Oxygenase,HO-1)和胆绿素还原酶A(BLVRA)为目的基因,选择GAPDH作为参照基因,通过实时荧光定量PCR,分析HO-1和BLVRA在不同品种鸡的机体内转录水平的差异。结果表明:卢氏鸡子宫部组织中HO-1的m RNA相对表达量显著高于固始鸡(P<0.05)。固始鸡子宫部组织中BLVRA m RNA相对表达量极显著高于卢氏鸡(P<0.01)。
译  名：
The Effects of the Expression Levels of H0-1 and BLVRA on the Color of Egg Shells
作  者：
Wang Xiaoqing;Xu Tingsheng;Lei Xueqin;Gao Lingzhao;Xiao Zhanqi;College of Animal Science and Technology, Henan University of Science and Technology;Lushi County Animal Husbandry Bureau;
关键词：
chicken;;HO-1;;BLVRA;;Real-time PCR;;egg shell color
摘  要：
This study aims to detect the expression difference of HO-1 and BLVRA in,uterine part of Lushichickens and Gushi chickens, thus discover the formation mechanism of green eggs. To conduct theexperiment, Lushi chickens laying green eggs and Gushi chickens laying brown eggs, all of which were 24-week aged, were randomly selected and the uterus of each chicken was collected. Then the total RNA wasextracted and later reversely transcribed into c DNA. The pigment-associated genes, HO-1 and BLVRA werechosen to be the target genes, and GAPDH the reference genes. Finally, the transcription levels of HO-1 andBLVRA in different breeds of chickens were analyzed using real- time fluorescent quantitation PCR. Theresults showed that the HO-1 m RNA expression level in the uterus of Lushi chickens was higher than that ofGushi chickens(P<0.05), and that the BLVRA m RNA expression level in the uterus of Gushi chickens wassignificantly higher than that of Lushi chickens(P<0.01).

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