单 位：
农业部淡水生物多样性保护与利用重点开放实验室
关键词：
中华沙鳅(Botia superciliaris);遗传多样性;线粒体DNA控制区;长江上游
摘 要：
测定了采自3个地理位置(宜宾、江津、思南)共45尾中华沙鳅(Botia superciliaris)的线粒体DNA控制区序列。结果显示:在所有个体913 bp序列中检测到22个变异位点,45尾个体中发现26种单倍型。中华沙鳅的平均核苷酸多样性指数π较低(0.00365),而平均单倍型多样性指数Hd较高(0.986)。中性检验Fu′sFs值为-9.49536(P<0.05)。3个群体内的Kimura 2-paramter遗传距离为0.00329～0.00417,群体间为0.00340～0.00379,群体间和群体内的遗传距离处于同一水平。分子变异分析(AMOVA)结果显示:Fst=0.0291(P<0.01),2.9%的变异来自群体间,97.1%的变异来自群体内;在NJ聚类树中,3个群体的个体并没有按相应的地理位置进行聚类。
译 名：
Genetic diversity of Botia superciliaris in the upper Yangtze River
作 者：
LIU Hong-yan1,2,CHEN Da-qing1,LIU Shao-ping1,DUAN Xin-bin1,3(1.Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000;2.School of Life Sciences,Jianghan University,Wuhan 430056;3.Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi,Jiangsu 214081)
关键词：
Botia superciliaris;genetic diversity;mtDNA control region;upper Yangtze River
摘 要：
To know the genetic diversity and differentiation of Botia superciliaris,the mitochondrial DNA control region was amplified and sequenced for 45 individuals from three regions of the upper Yangtze River,including Yibin,Jiangjin and Sinan.Altogether 22 variable nucleotide sites existed among the aligned sequences of 913 bp,and 26 haplotypes were found within 45 individuals.The average nucleotide diversity(π) of B.superciliaris was low(0.00365),while haplotype diversity(Hd) was high(0.986).Fu′s Fs of neutrality tests was-9.49534(P<0.05).Kimura 2-paramter genetic distance was 0.00329~0.00417 within the three populations,and was 0.00340~0.00379 among the three populations,and the two genetic distances seemed in the same level.AMOVA analysis demonstrated that Fst=0.0291(P<0.01),small variances occurred among populations(2.9%) and most variances occurred within populations(97.1%).Phylogenetic tree estimated by NJ methods indicated that the phylogenetic relationship of the B.superciliaris individuals was not correlative to their geographical locations.
