中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 大肠杆菌质粒与喹诺酮耐药性关系的研究 吉林农业大学学报 2001,23 (2) 94-97
Position: Home > Articles > Relation between Plasmids and Quinolone Resistance of Escherichia coli Journal of Jilin Agricultural University 2001,23 (2) 94-97

大肠杆菌质粒与喹诺酮耐药性关系的研究

作  者：
雷连成;韩文瑜;王兴龙;陈伟
单  位：
解放军军需大学动物科技系;新疆塔里木农垦大学动物科技学院
关键词：
大肠杆菌;质粒;喹诺酮;氧氟沙星;消除剂;耐药性
摘  要：
为研究大肠杆菌质粒与喹诺酮耐药性的关系 ,临床分离 32株耐喹诺酮药物的致病性大肠杆菌 ,经质粒消除剂溴化乙淀 (EB)、硫酸黄连素 (BS)分别处理 2 4h和 48h ,其中 4株对喹诺酮的耐药水平明显降低。经消除剂作用的菌株提取质粒进行电泳检测 ,结果只有大肠杆菌CE0 1[其中氧氟沙星 (OFL)的最小抑菌浓度(MIC) >5 0 μg/mL]的质粒带发生变化 ,且该质粒的转移与喹诺酮抗性有关。经消除剂BS作用 2 4h ,在含OFL 2 0 μg/mL的琼脂不能生长的CE0 1XC1菌株其质粒带浓度显著降低 ;作用 48h后 ,在含OFL 2 0 μg/mL琼脂不能生长的CE0 1XC2菌株其质粒带被消除 ;BS作用 48h后在含OFL 2 0 μg/mL琼脂仍然生长的CE0 1XC3菌株其质粒带几乎未改变。另外 3株耐药水平降低的菌株质粒带无变化。结果表明 :大肠杆菌CE0 1含有与喹诺酮药物抗性有关的质粒 ,这一质粒被消除剂消除后 ,CE0 1菌株的耐药性显著下降 ,对OFL的MIC由大于 5 0 μg/mL变为小于 2 0 μg/mL。其它 3株经质粒消除剂作用耐药水平降低的菌株可能是细胞膜通透性改变导致耐药。
译  名：
Relation between Plasmids and Quinolone Resistance of Escherichia coli
作  者：
LEI Lian cheng 1, HAN Wen yu 1, WANG Xing long 1, CHEN Wei 2 (1.Faculty of Animal Science and Technology, Quartermaster University of PLA, Jilin Changchun 130062, China; 2.College of Animal Science and Technology,Tarim Agriculture and Reclaimati
关键词：
Escherichia coli ; plasmid; quinolone; ofloxacin; eliminator; resistance
摘  要：
Thirty two ofloxacin resistant strains of chicken pathogenic E.coli were isolated from clinical samples. All strains were cultured in LB culture medium containing eliminator berberini sulfatis (BS) or ethidium bromide (EB) for 24 or 48 hours. The levels of quinolone resistance of four strains were clearly reduced after treatment. Plasmids of these four strains were extracted and tested with electrophoresis, only the plasmids of strain CE01, of which the transfer of plasmids was associated with quinolone resistance, had been eliminated by BS or EB, while there were no changes in plasmids after treated with eliminators in the others. Concentration of the plasmid electrophoretic band of CE01XC1, which could not grow in the culture medium containing ofloxacin 20?μg/mL, discreased significantly then the bacteria were treated with BS for 24?h; the band of CE01XC2 disappeared when they were cultured in the same medium as CE01XC1 for 48?h; the band concentration of CE01XC3, which grew well in the culture medium containing ofloxacin 20?μg/mL, hardly changed. The results suggest that strain CE01 has a plasmid which is associated with quinolone resistance.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46