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Position: Home > Articles > Rapid Extraction of Total RNA from Capsicum annuum Leaves Hubei Agricultural Sciences 2011,50 (17) 183-184

辣椒叶片总RNA快速提取

作  者:
李小霞;肖仲久;宋培勇;周逊;谢语
单  位:
遵义师范学院生物系
关键词:
辣椒(Capsicum annuum);总RNA;改良Trizol法;提取
摘  要:
采用改良的Trizol法对辣椒(Capsicum annuum)叶片的总RNA进行了提取,利用琼脂糖凝胶电泳、紫外分光光度法、RT-PCR进行RNA纯度、完整性检测。结果表明,Trizol法提取可获得较高质量的辣椒叶片总RNA,能满足后续的研究需要。
译  名:
Rapid Extraction of Total RNA from Capsicum annuum Leaves
作  者:
LI Xiao-xia,XIAO Zhong-jiu,SONG Pei-yong,ZHOU Xun,XIE Yu(Biology Department,Zunyi Normal College,Zunyi 563002,Guizhou,China)
关键词:
Capsicum annuum;total RNA;modified Trizol method;extraction
摘  要:
Trizol extraction method was modified to extract total RNA from Capsicum annuum leaves.The result of agar gel electrophoresis,ultraviolet ray photometer and RT-PCR showed that the total RNA obtained by modified Trizol method was of high quality,and suitable for downstream applications.

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